Development and Application of NanoPCR Method for Detection of Feline Panleukopenia Virus

Overview

Journal Vet Sci

Publisher MDPI

Specialty Veterinary Medicine

Date 2023 Jul 28

PMID 37505845

Authors

Haowen Xue

Yang Liang

Xu Gao

Yanhao Song

Kunru Zhu

Meng Yang

Jingrui Hao

Haoyuan Ma

Kai Yu

Affiliations

Soon will be listed here.

Abstract

Feline panleukopenia (FP) is a severe viral illness caused by the feline panleukopenia virus (FPV), putting sectors like companion cat breeding and endangered feline conservation at risk. The virus has a high morbidity and fatality rate and is found all over the world. We created a novel FPV assay using nanoPCR technology and assessed the method's specificity and sensitivity. The approach amplified a 345 bp nucleic acid fragment with a minimum detection limit of 7.97 × 10 copies/μL, which is about 100 times greater than traditional PCR. We collected anal swabs from 83 cats suspected of FPV infection for practical application, and the FPV-positive rate determined by the nanoPCR approach was 77.1%. In conclusion, the approach is more sensitive than conventional PCR and more convenient and cost-effective than qPCR methodology and may be utilized for the clinical detection of FPV.

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