Alveolar Bone Preservation Using a Combination of Nanocrystalline Hydroxyapatite and Injectable Platelet-Rich Fibrin: A Study in Rats

Overview

Journal Curr Issues Mol Biol

Publisher MDPI

Specialty Molecular Biology

Date 2023 Jul 28

PMID 37504293

Authors

Andries Pascawinata

Gusti Revilla

Roni Eka Sahputra

Syukri Arief

Affiliations

Soon will be listed here.

Abstract

Alveolar bone resorption is a post-extraction complication wherein there is a reduction in the dimensions and quality of the alveolar bone. This study aimed to examine the effects of implantation of a combination of nanocrystalline hydroxyapatite (nHA) and injectable platelet-rich fibrin (IPRF) on the expression of tartrate-resistant acid phosphatase (TRAP), alkaline phosphatase (ALP), osteocalcin (OCN), and new bone formation. A total of 32 male rats had their upper right incisors extracted under general anesthesia and were then divided into a control group, nHA group, IPRF group, and nHA-IPRF group. Decapitation was carried out on day 14 and day 28 in each group and the jaws of each rat were subjected to immunohistochemical and histological analysis. The results showed a decrease in TRAP expression in the nHA-IPRF group compared with the control group on day 14 ( = 0.074) and day 28 ( = 0.017). The study also showed an increase in ALP and OCN in the HA-IPRF group on day 14 and day 28 compared with the control group. New bone formation suggested a significant increase in the nHA-IPRF group compared with the control group on day 14 ( = 0.001) and day 28 ( = 0.001). nHA-IPRF implantation can suppress alveolar bone resorption, which is indicated by decreased TRAP expression, and it can increase bone growth, as indicated by increased expression of ALP, OCN, and new bone formation.

Citing Articles

Navigating the combinations of platelet-rich fibrin with biomaterials used in maxillofacial surgery.

Ievina L, Dubnika A Front Bioeng Biotechnol. 2024; 12:1465019.

PMID: 39434715 PMC: 11491360. DOI: 10.3389/fbioe.2024.1465019.

References

Rakhmatia Y, Ayukawa Y, Furuhashi A, Koyano K . Carbonate Apatite Containing Statin Enhances Bone Formation in Healing Incisal Extraction Sockets in Rats. Materials (Basel). 2018; 11(7). PMC: 6073205. DOI: 10.3390/ma11071201. View

Mohd Zaffarin A, Ng S, Ng M, Hassan H, Alias E . Nano-Hydroxyapatite as a Delivery System for Promoting Bone Regeneration In Vivo: A Systematic Review. Nanomaterials (Basel). 2021; 11(10). PMC: 8538947. DOI: 10.3390/nano11102569. View

Wang C, Yu S, Fretwurst T, Larsson L, Sugai J, Oh J . Maresin 1 Promotes Wound Healing and Socket Bone Regeneration for Alveolar Ridge Preservation. J Dent Res. 2020; 99(8):930-937. PMC: 7338694. DOI: 10.1177/0022034520917903. View

Farshidfar N, Jafarpour D, Firoozi P, Sahmeddini S, Hamedani S, de Souza R . The application of injectable platelet-rich fibrin in regenerative dentistry: A systematic scoping review of and studies. Jpn Dent Sci Rev. 2022; 58:89-123. PMC: 8971935. DOI: 10.1016/j.jdsr.2022.02.003. View

Boanini E, Torricelli P, Sima F, Axente E, Fini M, Mihailescu I . Gradient coatings of strontium hydroxyapatite/zinc β-tricalcium phosphate as a tool to modulate osteoblast/osteoclast response. J Inorg Biochem. 2018; 183:1-8. DOI: 10.1016/j.jinorgbio.2018.02.024. View

Thanasrisuebwong P, Surarit R, Bencharit S, Ruangsawasdi N . Influence of Fractionation Methods on Physical and Biological Properties of Injectable Platelet-Rich Fibrin: An Exploratory Study. Int J Mol Sci. 2019; 20(7). PMC: 6479392. DOI: 10.3390/ijms20071657. View

Liu Z, Jin H, Xie Q, Jiang Z, Guo S, Li Y . Controlled Release Strategies for the Combination of Fresh and Lyophilized Platelet-Rich Fibrin on Bone Tissue Regeneration. Biomed Res Int. 2019; 2019:4923767. PMC: 6541981. DOI: 10.1155/2019/4923767. View

Suarez-Lopez Del Amo F, Monje A . Efficacy of biologics for alveolar ridge preservation/reconstruction and implant site development: An American Academy of Periodontology best evidence systematic review. J Periodontol. 2022; 93(12):1827-1847. PMC: 10092438. DOI: 10.1002/JPER.22-0069. View

Stumbras A, Kuliesius P, Januzis G, Juodzbalys G . Alveolar Ridge Preservation after Tooth Extraction Using Different Bone Graft Materials and Autologous Platelet Concentrates: a Systematic Review. J Oral Maxillofac Res. 2019; 10(1):e2. PMC: 6498816. DOI: 10.5037/jomr.2019.10102. View

10.

Fedchenko N, Reifenrath J . Different approaches for interpretation and reporting of immunohistochemistry analysis results in the bone tissue - a review. Diagn Pathol. 2014; 9:221. PMC: 4260254. DOI: 10.1186/s13000-014-0221-9. View

11.

Calasans-Maia M, Barboza Junior C, Soriano-Souza C, Alves A, Uzeda M, Martinez-Zelaya V . Microspheres of alginate encapsulated minocycline-loaded nanocrystalline carbonated hydroxyapatite: therapeutic potential and effects on bone regeneration. Int J Nanomedicine. 2019; 14:4559-4571. PMC: 6600321. DOI: 10.2147/IJN.S201631. View

12.

Karde P, Sethi K, Mahale S, Khedkar S, Patil A, Joshi C . Comparative evaluation of platelet count and antimicrobial efficacy of injectable platelet-rich fibrin with other platelet concentrates: An study. J Indian Soc Periodontol. 2018; 21(2):97-101. PMC: 5771122. DOI: 10.4103/jisp.jisp_201_17. View

13.

de Sousa C, Lemos C, Santiago-Junior J, Faverani L, Pellizzer E . Bone augmentation using autogenous bone versus biomaterial in the posterior region of atrophic mandibles: A systematic review and meta-analysis. J Dent. 2018; 76:1-8. DOI: 10.1016/j.jdent.2018.06.014. View

14.

Mallappa J, Vasanth D, Gowda T, Shah R, Gayathri G, Mehta D . Clinicoradiographic evaluation of advanced-platelet rich fibrin block (A PRF + i PRF + nanohydroxyapatite) compared to nanohydroxyapatite alone in the management of periodontal intrabony defects. J Indian Soc Periodontol. 2022; 26(4):359-364. PMC: 9362812. DOI: 10.4103/jisp.jisp_882_20. View

15.

Xu G, Shen C, Lin H, Zhou J, Wang T, Wan B . Development, Characterization and Osteoinductive Efficacy of a Novel Biomimetically-Precipitated Nanocrystalline Calcium Phosphate With Internally-Incorporated Bone Morphogenetic Protein-2. Front Bioeng Biotechnol. 2022; 10:920696. PMC: 9354744. DOI: 10.3389/fbioe.2022.920696. View

16.

Sculean A, Stavropoulos A, Bosshardt D . Self-regenerative capacity of intra-oral bone defects. J Clin Periodontol. 2019; 46 Suppl 21:70-81. DOI: 10.1111/jcpe.13075. View

17.

Mu Z, Chen K, Yuan S, Li Y, Huang Y, Wang C . Gelatin Nanoparticle-Injectable Platelet-Rich Fibrin Double Network Hydrogels with Local Adaptability and Bioactivity for Enhanced Osteogenesis. Adv Healthc Mater. 2020; 9(5):e1901469. DOI: 10.1002/adhm.201901469. View

18.

Alhasyimi A, Pudyani P, Asmara W, Ana I . Enhancement of post-orthodontic tooth stability by carbonated hydroxyapatite-incorporated advanced platelet-rich fibrin in rabbits. Orthod Craniofac Res. 2018; 21(2):112-118. DOI: 10.1111/ocr.12224. View

19.

Albeshri S, Alblaihess A, Niazy A, Ramalingam S, Sundar C, Alghamdi H . Biomarkers as Independent Predictors of Bone Regeneration around Biomaterials: A Systematic Review of Literature. J Contemp Dent Pract. 2018; 19(5):605-618. View

20.

Canellas J, Ritto F, Figueredo C, Fischer R, de Oliveira G, Thole A . Histomorphometric evaluation of different grafting materials used for alveolar ridge preservation: a systematic review and network meta-analysis. Int J Oral Maxillofac Surg. 2019; 49(6):797-810. DOI: 10.1016/j.ijom.2019.10.007. View