Potential Clinical Application of Organs-on-a-Chip in Periodontal Diseases: A Systematic Review of In Vitro Studies

Overview

Journal Dent J (Basel)

Specialty Dentistry

Date 2023 Jul 28

PMID 37504224

Authors

Carlos M Ardila

Gustavo A Jimenez-Arbelaez

Annie Marcela Vivares-Builes

Affiliations

Soon will be listed here.

Abstract

The periodontium is a unique organ from the standpoint of building an organ-on-a-chip (OoC) since it is a system that is continually threatened by microorganisms, their noxious compounds, and antigenic components. At the same time, periodontal health depends on a balanced connection between the host and the bacteria in the oral cavity, which is a complex micro-ecological environment. The objective of this systematic review of in vitro studies is to revise the potential clinical application of OoC in periodontal diseases. PRISMA was used to guide this analysis. The review framework made use of several databases, including SCOPUS, PubMed/MEDLINE, SCIELO, and LILACS as well as the gray literature. This systematic review comprised seven studies. The clinical efficacy of OoC in periodontal diseases was observed in models of the gingival crevice for the research of periodontitis, periodontal medication analysis, the interaction of multiple microbial species, pH measurements in in situ-grown biofilm, testing antimicrobial reagents, evaluation of mucosal interactions with microorganisms, and a device for quantitative exploration of microorganisms. OoC has the potential to advance our understanding of periodontal diseases by providing a more accurate representation of the oral microenvironment and enabling the development of new treatments.

Citing Articles

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References

Baptista L, Porrini C, Kronemberger G, Kelly D, Perrault C . 3D organ-on-a-chip: The convergence of microphysiological systems and organoids. Front Cell Dev Biol. 2022; 10:1043117. PMC: 9720174. DOI: 10.3389/fcell.2022.1043117. View

Ehsani S, Mandich M, El-Bialy T, Flores-Mir C . Frictional resistance in self-ligating orthodontic brackets and conventionally ligated brackets. A systematic review. Angle Orthod. 2009; 79(3):592-601. DOI: 10.2319/060208-288.1. View

Orlandi M, Munoz Aguilera E, Marletta D, Petrie A, Suvan J, DAiuto F . Impact of the treatment of periodontitis on systemic health and quality of life: A systematic review. J Clin Periodontol. 2021; 49 Suppl 24:314-327. DOI: 10.1111/jcpe.13554. View

Luo T, Hayashi M, Zsiska M, Circello B, Eisenberg M, Gonzalez-Cabezas C . Introducing BAIT (Biofilm Architecture Inference Tool): a software program to evaluate the architecture of oral multi-species biofilms. Microbiology (Reading). 2019; 165(5):527-537. DOI: 10.1099/mic.0.000761. View

Zhang M, Wang P, Luo R, Wang Y, Li Z, Guo Y . Biomimetic Human Disease Model of SARS-CoV-2-Induced Lung Injury and Immune Responses on Organ Chip System. Adv Sci (Weinh). 2020; 8(3):2002928. PMC: 7646023. DOI: 10.1002/advs.202002928. View

Dama A, Kim K, Leyva D, Lunkes A, Schmid N, Jijakli K . BacterAI maps microbial metabolism without prior knowledge. Nat Microbiol. 2023; 8(6):1018-1025. DOI: 10.1038/s41564-023-01376-0. View

Engen S, Rorvik G, Schreurs O, Blix I, Schenck K . The oral commensal Streptococcus mitis activates the aryl hydrocarbon receptor in human oral epithelial cells. Int J Oral Sci. 2017; 9(3):145-150. PMC: 5709542. DOI: 10.1038/ijos.2017.17. View

Bao K, Belibasakis G, Selevsek N, Grossmann J, Bostanci N . Proteomic profiling of host-biofilm interactions in an oral infection model resembling the periodontal pocket. Sci Rep. 2015; 5:15999. PMC: 4630604. DOI: 10.1038/srep15999. View

Hwang G, Liu Y, Kim D, Sun V, Aviles-Reyes A, Kajfasz J . Simultaneous spatiotemporal mapping of in situ pH and bacterial activity within an intact 3D microcolony structure. Sci Rep. 2016; 6:32841. PMC: 5015094. DOI: 10.1038/srep32841. View

10.

Lagosz-Cwik K, Wielento A, Lipska W, Kantorowicz M, Darczuk D, Kaczmarzyk T . hTERT-immortalized gingival fibroblasts respond to cytokines but fail to mimic primary cell responses to Porphyromonas gingivalis. Sci Rep. 2021; 11(1):10770. PMC: 8144196. DOI: 10.1038/s41598-021-90037-5. View

11.

Stolte K, Pelz C, Yapto C, Raguse J, Dommisch H, Danker K . IL-1β strengthens the physical barrier in gingival epithelial cells. Tissue Barriers. 2020; 8(3):1804249. PMC: 7549743. DOI: 10.1080/21688370.2020.1804249. View

12.

Shang L, Cheng Y, Wang J, Ding H, Rong F, Zhao Y . Double emulsions from a capillary array injection microfluidic device. Lab Chip. 2014; 14(18):3489-93. DOI: 10.1039/c4lc00698d. View

13.

Jin L, Kou N, An F, Gao Z, Tian T, Hui J . Analyzing Human Periodontal Soft Tissue Inflammation and Drug Responses In Vitro Using Epithelium-Capillary Interface On-a-Chip. Biosensors (Basel). 2022; 12(5). PMC: 9138963. DOI: 10.3390/bios12050345. View

14.

Sriram G, Natu V, Islam I, Fu X, Seneviratne C, Tan K . Innate Immune Response of Human Embryonic Stem Cell-Derived Fibroblasts and Mesenchymal Stem Cells to Periodontopathogens. Stem Cells Int. 2016; 2016:8905365. PMC: 5014959. DOI: 10.1155/2016/8905365. View

15.

Zheng F, Fu F, Cheng Y, Wang C, Zhao Y, Gu Z . Organ-on-a-Chip Systems: Microengineering to Biomimic Living Systems. Small. 2016; 12(17):2253-82. DOI: 10.1002/smll.201503208. View

16.

Rahimi C, Rahimi B, Padova D, Rooholghodos S, Bienek D, Luo X . Oral mucosa-on-a-chip to assess layer-specific responses to bacteria and dental materials. Biomicrofluidics. 2018; 12(5):054106. PMC: 6158033. DOI: 10.1063/1.5048938. View

17.

Friesen L, Goyal C, Qaqish J, He T, Eusebio R, Zsiska M . Comparative Antiplaque Effect of Two Antimicrobial Dentifrices: Laboratory and Clinical Evaluations. J Clin Dent. 2018; 28(4 Spec No B):B6-11. View

18.

Zou H, Zhou N, Cheng X, Qiu Y, Hou W, Sun J . Gingipains are the important virulence factors of Porphyromonas gingivalis downregulating B10 cells. Mol Oral Microbiol. 2023; 38(4):275-288. DOI: 10.1111/omi.12413. View

19.

Delon L, Nilghaz A, Cheah E, Prestidge C, Thierry B . Unlocking the Potential of Organ-on-Chip Models through Pumpless and Tubeless Microfluidics. Adv Healthc Mater. 2020; 9(11):e1901784. DOI: 10.1002/adhm.201901784. View

20.

Luthra S, Orlandi M, Hussain S, Leira Y, Botelho J, Machado V . Treatment of periodontitis and C-reactive protein: A systematic review and meta-analysis of randomized clinical trials. J Clin Periodontol. 2022; 50(1):45-60. PMC: 10087558. DOI: 10.1111/jcpe.13709. View