Endometriosis-targeted MRI Imaging Using Bevacizumab-modified Nanoparticles Aimed at Vascular Endothelial Growth Factor

Overview

Journal Nanoscale Adv

Publisher Royal Society of Chemistry

Specialty Biotechnology

Date 2023 Jul 27

PMID 37496625

Authors

Qi Zhang

Shiman Wu

Yajie Li

Mao Lai

Qing Li

Caixia Fu

Zhenwei Yao

Junhai Zhang

Affiliations

Soon will be listed here.

Abstract

Endometriosis is a tumor-like disease with high recurrence. In this case, the accurate imaging-based diagnosis of endometriosis can help clinicians eradicate it by improving their surgical plan. However, although contrast agents can improve the visibility of the tissue of interest magnetic resonance imaging (MRI), the lack of biomarkers in endometriosis hinders the development of agents for its targeted imaging and diagnosis. Herein, aiming at the enriched vascular endothelial growth factor (VEGF) in endometriosis, we developed a targeting MRI contrast agent modified with bevacizumab, , NaGdF@PEG@bevacizumab-Cy5.5 nanoparticles (NPBCNs), to detect endometriosis. NPBCNs showed negligible cytotoxicity and high affinity towards VEGF in endometrial cells . Furthermore, NPBCNs generated a strong signal enhancement in endometriosis lesions in rats in -weighted images MRI at 3 days post-injection, as confirmed by the histopathological staining results and fluorescence imaging on the same day. Our approach can enable NPBCNs to target endometriosis effectively, thus avoiding missed diagnoses.

Citing Articles

The Role of Nanomedicine in Benign Gynecologic Disorders.

Lulseged B, Ramaiyer M, Michel R, Saad E, Ozpolat B, Borahay M Molecules. 2024; 29(9).

PMID: 38731586 PMC: 11085148. DOI: 10.3390/molecules29092095.

References

Maeda H, Wu J, Sawa T, Matsumura Y, Hori K . Tumor vascular permeability and the EPR effect in macromolecular therapeutics: a review. J Control Release. 2000; 65(1-2):271-84. DOI: 10.1016/s0168-3659(99)00248-5. View

Ohashi N, Aoki R, Shinozaki S, Naito N, Ohyama K . A case of anemia with schistocytosis, thrombocytopenia, and acute renal failure caused by adenomyosis. Intern Med. 2011; 50(20):2347-50. DOI: 10.2169/internalmedicine.50.5628. View

Guo S . Recurrence of endometriosis and its control. Hum Reprod Update. 2009; 15(4):441-61. DOI: 10.1093/humupd/dmp007. View

Farquhar C . Endometriosis. BMJ. 2007; 334(7587):249-53. PMC: 1790744. DOI: 10.1136/bmj.39073.736829.BE. View

Erogbogbo F, Chang C, May J, Liu L, Kumar R, Law W . Bioconjugation of luminescent silicon quantum dots to gadolinium ions for bioimaging applications. Nanoscale. 2012; 4(17):5483-9. DOI: 10.1039/c2nr31002c. View

Ni D, Jiang D, Im H, Valdovinos H, Yu B, Goel S . Radiolabeled polyoxometalate clusters: Kidney dysfunction evaluation and tumor diagnosis by positron emission tomography imaging. Biomaterials. 2018; 171:144-152. PMC: 5962344. DOI: 10.1016/j.biomaterials.2018.04.019. View

Kralickova M, Lagana A, Ghezzi F, Vetvicka V . Endometriosis and risk of ovarian cancer: what do we know?. Arch Gynecol Obstet. 2019; 301(1):1-10. DOI: 10.1007/s00404-019-05358-8. View

Ovais M, Mukherjee S, Pramanik A, Das D, Mukherjee A, Raza A . Designing Stimuli-Responsive Upconversion Nanoparticles that Exploit the Tumor Microenvironment. Adv Mater. 2020; 32(22):e2000055. DOI: 10.1002/adma.202000055. View

Suk J, Xu Q, Kim N, Hanes J, Ensign L . PEGylation as a strategy for improving nanoparticle-based drug and gene delivery. Adv Drug Deliv Rev. 2015; 99(Pt A):28-51. PMC: 4798869. DOI: 10.1016/j.addr.2015.09.012. View

10.

Zhang D, Zhuang R, Guo Z, Gao M, Huang L, You L . Desmin- and vimentin-mediated hepatic stellate cell-targeting radiotracer Tc-GlcNAc-PEI for liver fibrosis imaging with SPECT. Theranostics. 2018; 8(5):1340-1349. PMC: 5835940. DOI: 10.7150/thno.22806. View

11.

Zhang M, Wang X, Xia X, Fang X, Zhang T, Huang F . Endometrial epithelial cells-derived exosomes deliver microRNA-30c to block the BCL9/Wnt/CD44 signaling and inhibit cell invasion and migration in ovarian endometriosis. Cell Death Discov. 2022; 8(1):151. PMC: 8976844. DOI: 10.1038/s41420-022-00941-6. View

12.

Kamrani S, Amirchaghmaghi E, Ghaffari F, Shahhoseini M, Ghaedi K . Altered gene expression of VEGF, IGFs and H19 lncRNA and epigenetic profile of H19-DMR region in endometrial tissues of women with endometriosis. Reprod Health. 2022; 19(1):100. PMC: 9034598. DOI: 10.1186/s12978-022-01406-w. View

13.

Kvaskoff M, Mahamat-Saleh Y, Farland L, Shigesi N, Terry K, Harris H . Endometriosis and cancer: a systematic review and meta-analysis. Hum Reprod Update. 2020; 27(2):393-420. DOI: 10.1093/humupd/dmaa045. View

14.

Giudice L, Kao L . Endometriosis. Lancet. 2004; 364(9447):1789-99. DOI: 10.1016/S0140-6736(04)17403-5. View

15.

Liu Z, Zhao M, Wang H, Fu Z, Gao H, Peng W . High relaxivity Gd-based organic nanoparticles for efficient magnetic resonance angiography. J Nanobiotechnology. 2022; 20(1):170. PMC: 8973627. DOI: 10.1186/s12951-022-01363-3. View

16.

Fukui A, Mai C, Saeki S, Yamamoto M, Takeyama R, Kato T . Pelvic endometriosis and natural killer cell immunity. Am J Reprod Immunol. 2020; 85(4):e13342. DOI: 10.1111/aji.13342. View

17.

Peng B, Alotaibi F, Sediqi S, Bedaiwy M, Yong P . Role of interleukin-1β in nerve growth factor expression, neurogenesis and deep dyspareunia in endometriosis. Hum Reprod. 2020; 35(4):901-912. PMC: 7192531. DOI: 10.1093/humrep/deaa017. View

18.

Xing H, Zhang S, Bu W, Zheng X, Wang L, Xiao Q . Ultrasmall NaGdF4 nanodots for efficient MR angiography and atherosclerotic plaque imaging. Adv Mater. 2014; 26(23):3867-72. DOI: 10.1002/adma.201305222. View

19.

Zhang H, Wu Y, Wang J, Tang Z, Ren Y, Ni D . In Vivo MR Imaging of Glioma Recruitment of Adoptive T-Cells Labeled with NaGdF -TAT Nanoprobes. Small. 2017; 14(3). DOI: 10.1002/smll.201702951. View

20.

Carlo C, Bonifacio M, Tommaselli G, Bifulco G, Guerra G, Nappi C . Metalloproteinases, vascular endothelial growth factor, and angiopoietin 1 and 2 in eutopic and ectopic endometrium. Fertil Steril. 2008; 91(6):2315-23. DOI: 10.1016/j.fertnstert.2008.03.079. View