Efficient Splicing of the CPE Intein Derived from Directed Evolution of the PRP8 Intein

Overview

Journal Acta Biochim Biophys Sin (Shanghai)

Specialties Biochemistry
Biophysics

Date 2023 Jul 25

PMID 37489009

Authors

Qin Zhan

Changhua Shi

Yu Jiang

Xianling Gao

Ying Lin

Affiliations

Soon will be listed here.

Abstract

Intein-mediated protein splicing has been widely used in protein engineering; however, the splicing efficiency and extein specificity usually limit its further application. Thus, there is a demand for more general inteins that can overcome these limitations. Here, we study the -splicing of CPE intein obtained from the directed evolution of PRP8, which shows that its splicing rate is ~29- higher than that of the wild-type. When the +1 residue of C-extein is changed to cysteine, CPE also shows high splicing activity. Faster association and higher affinity may contribute to the high splicing rate compared with wild-type intein. These findings have important implications for the future engineering of inteins and provide clues for fundamental studies of protein structure and folding.

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