Ultrastructure of Reconstituted Membranes Containing the Muscarinic Receptor

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1986 Jul 1

PMID 3748279

Citations 1

Authors

A PELLEGRINO DE IRALDI

J Santiago Aguilar

E L Ochoa

Affiliations

Soon will be listed here.

Abstract

In this paper the demonstration is made that membrane vesicles (liposomes) containing the muscarinic receptor can be formed by polyethylene glycol (PEG) precipitation of detergent extracts of bovine atrial membranes. The incorporation of the muscarinic receptor in these vesicles may be related to the restoration of the heterogeneity and nucleotide modulation of muscarinic agonist binding by PEG precipitation of atrial detergent extracts, previously reported. Vesicles are also formed when detergent solubilized asolectin lipids, alone or in combination with membrane detergent extracts, are precipitated by PEG. The structure of the vesicles seems depend on their lipid and protein composition and the procedure employed for the removal of the dispersing medium. These results indicate that PEG precipitation could be used for the reconstitution of the muscarinic receptor into the liposomes of exogenous lipids.

Citing Articles

Reconstitution of solubilized atrial cholinergic muscarinic receptors in liposomes.

Aguilar J, Ochoa E, De Robertis E Neurochem Res. 1987; 12(1):83-91.

PMID: 3574589 DOI: 10.1007/BF00971369.

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