Single-cell Genomics in Acquired Bone Marrow Failure Syndromes

Overview

Journal Blood

Publisher Elsevier

Specialty Hematology

Date 2023 Jul 21

PMID 37478398

Authors

Zhijie Wu

Neal S Young

Affiliations

Soon will be listed here.

Abstract

Mechanistic studies of immune bone marrow failure are difficult because of the scarcity of residual cells, the involvement of multiple cell types, and the inherent complexities of hematopoiesis and immunity. Single-cell genomic technologies and bioinformatics allow extensive, multidimensional analysis of a very limited number of cells. We review emerging applications of single-cell techniques, and early results related to disease pathogenesis: effector and target cell populations and relationships, cell-autonomous and nonautonomous phenotypes in clonal hematopoiesis, transcript splicing, chromosomal abnormalities, and T-cell receptor usage and clonality. Dense and complex data from single-cell techniques provide insights into pathophysiology, natural history, and therapeutic drug effects.

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