Interactions of Arene Ruthenium(II) Complexes [η-(CH)Ru(pprip)Cl] and [η-(CH)Ru(HiiP)Cl] with RNA Triplex Poly(U)•poly(A)*poly(U)

Overview

Journal J Biol Inorg Chem

Publisher Springer

Specialty Biochemistry

Date 2023 Jul 21

PMID 37477757

Authors

Feng Yuan

Xiaohua Liu

Juan Li

Lifeng Tan

Affiliations

Soon will be listed here.

Abstract

Two arene ruthenium(II) complexes [η-(CH)Ru(pprip)Cl]PF (Ru1; pprip = 2-(3-phenyl-1H-pyrazol-4-yl)-imidazolo[4,5-f][1,10]phenanthroline) and [η-(CH)Ru(HiiP)Cl]PF (Ru2; HiiP = 2-(indole-3-yl)-imidazolo[4,5-f][1,10]phenanthroline) have been synthesized and characterized in this work. Binding properties of Ru1 and Ru2 with the triplex RNA poly(U)•poly(A)*poly(U) were investigated by spectrophotometry and spectrofluorometry as well as viscosimetry. Analysis of spectroscopic titrations and viscosity measurements show that the two complexes bind with the triplex through intercalation, while the binding affinity for Ru2 toward the triplex is stronger than that for Ru1. Melting experiments indicate that the stabilizing effects of Ru1 and Ru2 toward the triplex differ from each other. Under the conditions used herein, Ru1 only stabilizes the Hoogsteen base-paired strand (third strand) without affecting stabilization of the Watson-Crick base-paired strand (the template duplex) of the triplex, while Ru2 stabilizes both the template duplex and the third strand. Although the two complexes prefer to stabilizing the third strand rather than the template duplex, the third-strand stabilization effect of Ru2 is stronger than that of Ru1. The obtained results of this work reveal that the planarity of the intercalative ligands plays an important role in the triplex stabilization by arene Ru(II) complexes.

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