Anti- Activity of L. and Its Fractions: and Assays

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Jul 21

PMID 37476671

Authors

Shahira A Ahmed

Enas E Eltamany

Mohamed S Nafie

Sameh S Elhady

Panagiotis Karanis

Amira B Mokhtar

Affiliations

Soon will be listed here.

Abstract

Background: This study investigates the toxic activity of ethanolic extract (ArEx) as well as its phenolic fraction (ArPh), and terpenoid fraction (ArT) against () oocysts.

Methods: Over a 4 months period, estimation of the total phenolic (TPC), total flavonoids (TFC), and total terpenoids contents (TTC) in ArEx; investigation of the antioxidant activity of ArEx, ArPh, and ArT; evaluation of ArEx, ArPh, and ArT toxic activity against oocysts using MTT assay; parasitological analysis on ArPh-treated oocysts and comet assay were performed both and (infectivity).

Results: The ArEx TPC, TFC, and TTC was 52.6 ± 3.1 mgGAE/g, 64.5 ± 3.1 mg QE/g, and 9.5 ± 1.1 mg Linol/g, respectively. Regarding the phytochemical antioxidant activity, the ArPh exhibited the highest antioxidant activity compared to the ArEx and ArT. The ArPh showed promising free radical scavenging activity of DPPH and ABTS with IC values of 47.27 ± 1.86 μg/mL and 66.89 ± 1.94 μg/mL, respectively. Moreover, the FRAP of ArPh was 2.97 ± 0.65 mMol Fe/g while its TAC was 46.23 ± 3.15 mg GAE/g. The ArPh demonstrated toxic activity against oocysts with a potent IC value of 31.6 μg/mL compared to ArT (promising) and ArEx (non-effective). ArPh parasitological analysis demonstrated MIC at 1000 μg/ml and effective oocysts destruction on count and morphology. ArPh fragmented oocysts nuclear DNA in comet assay. Beginning at 200 μg/mL, ArPh-treated oocysts did not infect mice.

Conclusion: To combat infection, the phenolic fraction of L. shows promise as an adjuvant therapy or as a source of potentially useful lead structures for drug discovery.

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