Herbarium Specimen Sequencing Allows Precise Dating of Xanthomonas Citri Pv. Citri Diversification History

Overview

Journal Nat Commun

Specialty Biology

Date 2023 Jul 20

PMID 37474518

Authors

Paola E Campos

Olivier Pruvost

Karine Boyer

Frederic Chiroleu

Thuy Trang Cao

Myriam Gaudeul

Claudia Baider

Timothy M A Utteridge

Nathalie Becker

Adrien Rieux

Lionel Gagnevin

Affiliations

Soon will be listed here.

Abstract

Herbarium collections are an important source of dated, identified and preserved DNA, whose use in comparative genomics and phylogeography can shed light on the emergence and evolutionary history of plant pathogens. Here, we reconstruct 13 historical genomes of the bacterial crop pathogen Xanthomonas citri pv. citri (Xci) from infected Citrus herbarium specimens. Following authentication based on ancient DNA damage patterns, we compare them with a large set of modern genomes to estimate their phylogenetic relationships, pathogenicity-associated gene content and several evolutionary parameters. Our results indicate that Xci originated in Southern Asia ~11,500 years ago (perhaps in relation to Neolithic climate change and the development of agriculture) and diversified during the beginning of the 13th century, after Citrus diversification and before spreading to the rest of the world (probably via human-driven expansion of citriculture through early East-West trade and colonization).

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