CaMKIIα Neurons in the Anterior Insular Cortex Regulate Attention Behavior in Mice

Overview

Journal Front Neural Circuits

Date 2023 Jul 20

PMID 37469833

Authors

Yingping Ma

Shaofei Jiang

Xin Zhao

Shen Li

Liping Chen

Zhe Zhao

Wei Shen

Yan Wu

Haitao Wu

Affiliations

Soon will be listed here.

Abstract

Introduction: The insular cortex is involved in multiple physiological processes including working memory, pain, emotion, and interoceptive functions. Previous studies have indicated that the anterior insular cortex (aIC) also mediates interoceptive attention in humans. However, the exact cellular and physiological function of the aIC in the regulation of this process is still elusive.

Methods: In this study, using the 5-choice serial reaction time task (5-CSRTT) testing paradigm, we assessed the role of the aIC in visuospatial attention and impulsiveness in mice.

Results: The results showed a dramatic activation of c-Fos in the aIC CaMKIIα neurons after the 5-CSRTT procedure. fiber photometry revealed enhanced calcium signaling in aIC CaMKIIα neurons when the mice responded correctly. In addition, chemogenetic suppression of aIC CaMKIIα neurons led to increased incorrect responses within the appropriate time. Importantly, pharmacological activation of aIC CaMKIIα neurons enhanced their performance in the 5-CSRTT test.

Discussion: These results provide compelling evidence that aIC CaMKIIα neurons are essential for the modulation of attentional processing in mice.

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