Molecular Characterization and Protective Efficacy of Vacuolar Protein Sorting 29 from

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2023 Jul 20

PMID 37469602

Authors

Tuan-Yuan Shi

Tian-En Li

Yun Hao

Hong-Chao Sun

Yuan Fu

Wen-Chao Yan

Li-Li Hao

Affiliations

Soon will be listed here.

Abstract

Introduction: Vacuolar protein sorting 29 (VPS29) is a core component of the retromer-retriever complex and is essential for recycling numerous cell-surface cargoes from endosomes. However, there are no reports yet on VPS29 of spp.

Methods: Here, we cloned and prokaryotically expressed a partial sequence of VPS29 (EtVPS29) with RT-PCR and engineered strain of respectively. The localization of the VPS29 protein in sporozoites was investigated with immunofluorescence (IFA) and overexpression assays. And its protective efficacy against infection was investigated in chickens with the animal protection test.

Results: An EtVPS29 gene fragment with an ORF reading frame of 549 bp was cloned. The band size of the expressed recombinant protein, rEtVPS29, was approximately 39 kDa and was recognized by the chicken anti- positive serum. EtVPS29 protein was observed widely distributing in the cytoplasm of sporozoites in the IFA and overexpression assays. rEtVPS29 significantly increased average body weight gain and decreased mean lesion score and oocyst output in chickens. The relative weight gain rate in the rEtVPS29-immunized group was 62.9%, which was significantly higher than that in the unimmunized and challenged group (P < 0.05). The percentage of reduced oocyst output in the rEtVPS29 immunized group was 32.2%. The anticoccidial index of the rEtVPS29-immunized group was 144.2. Serum ELISA also showed that rEtVPS29 immunization induced high levels of specific antibodies in chickens.

Discussion: These results suggest that rEtVPS29 can induce a specific immune response and is a potential candidate for the development of novel vaccines against infections in chickens.

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