Evaluation of IL-4, MIP-1α, and MMP-9 Gene Expression Levels in Peri-implant Tissues in Peri-implantitis

Overview

Journal Braz Dent J

Specialty Dentistry

Date 2023 Jul 19

PMID 37466520

Authors

Gabriela Giro

Jorge Taira

Fernando Andriani

Sidney Watinaga

Marta Ferreira Bastos

Jamil Awad Shibli

Affiliations

Soon will be listed here.

Abstract

This case-control study evaluated the gene expression levels of interleukin (IL)-4, macrophage inflammatory protein type 1 alpha (MIP-1α), and metalloproteinase (MMP)-9, factors involved in the formation of giant cells in healthy peri-implant tissue and peri-implantitis. Thirty-five subjects (15 healthy and 20 with peri-implantitis), who met the inclusion and exclusion criteria, were included in this study. The peri-implant tissue biopsies were subjected to total RNA extraction, DNAse treatment, and cDNA synthesis. Subsequently, the reaction of real-time PCR was performed to evaluate the gene expression levels of IL-4, MIP-1α, and MMP-9 concerning the reference gene. IL-4 gene expression showed higher (18-fold) values in the Peri-Implantitis Group of Patients when compared with the Healthy (Control) Group (p<0.0001). Although MIP- 1α and MMP-9 gene expression levels were higher in diseased implants, they showed no significant differences (p=0.06 and p=0.2337), respectively. Within the limitations of this study, the results showed that in tissues affected by peri-implantitis, only levels of Il-4 were increased when compared with tissues in the control group.

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