In Vitro Biological Responses of Plasma Nanocoatings for Coronary Stent Applications

Overview

Journal J Biomed Mater Res A

Specialty Biomedical Engineering

Date 2023 Jul 19

PMID 37465994

Authors

ThiThuHa Phan

John E Jones

Meng Chen

T L Strawn

Hekmat B Khoukaz

Yan Ji

Arun Kumar

Douglas K Bowles

William P Fay

Qingsong Yu

Affiliations

Soon will be listed here.

Abstract

In-stent restenosis and thrombosis remain to be long-term challenges in coronary stenting procedures. The objective of this study was to evaluate the in vitro biological responses of trimethylsilane (TMS) plasma nanocoatings modified with NH /O (2:1 molar ratio) plasma post-treatment (TMS + NH /O nanocoatings) on cobalt chromium (CoCr) alloy L605 coupons, L605 stents, and 316L stainless steel (SS) stents. Surface properties of the plasma nanocoatings with up to 2-year aging time were characterized by wettability assessment and x-ray photoelectron spectroscopy (XPS). It was found that TMS + NH /O nanocoatings had a surface composition of 41.21 ± 1.06 at% oxygen, 31.90 ± 1.08 at% silicon, and 24.12 ± 1.7 at% carbon, and very small but essential amount of 2.77 ± 0.18 at% nitrogen. Surface chemical stability of the plasma coatings was noted with persistent O/Si atomic ratio of 1.292-1.413 and N/Si atomic ratio of ~0.087 through 2 years. The in vitro biological responses of plasma nanocoatings were studied by evaluating the cell proliferation and migration of porcine coronary artery endothelial cells (PCAECs) and smooth muscle cells (PCASMCs). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay results revealed that, after 7-day incubation, TMS + NH /O nanocoatings maintained a similar level of PCAEC proliferation while showing a decrease in the viability of PCASMCs by 73 ± 19% as compared with uncoated L605 surfaces. Cell co-culture of PCAECs and PCASMCs results showed that, the cell ratio of PCAEC/PCASMC on TMS + NH /O nanocoating surfaces was 1.5-fold higher than that on uncoated L605 surfaces, indicating enhanced selectivity for promoting PCAEC growth. Migration test showed comparable PCAEC migration distance for uncoated L605 and TMS + NH /O nanocoatings. In contrast, PCASMC migration distance was reduced nearly 8.5-fold on TMS + NH /O nanocoating surfaces as compared to the uncoated L605 surfaces. Platelet adhesion test using porcine whole blood showed lower adhered platelets distribution (by 70 ± 16%), reduced clotting attachment (by 54 ± 12%), and less platelet activation on TMS + NH /O nanocoating surfaces as compared with the uncoated L605 controls. It was further found that, under shear stress conditions of simulated blood flow, TMS + NH /O nanocoating significantly inhibited platelet adhesion compared to the uncoated 316L SS stents and TMS nanocoated 316L SS stents. These results indicate that TMS + NH /O nanocoatings are very promising in preventing both restenosis and thrombosis for coronary stent applications.

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References

Szabo C, Ischiropoulos H, Radi R . Peroxynitrite: biochemistry, pathophysiology and development of therapeutics. Nat Rev Drug Discov. 2007; 6(8):662-80. DOI: 10.1038/nrd2222. View

Dewanjee M, Rao S, Didisheim P . Indium-111 tropolone, a new high-affinity platelet label: preparation and evaluation of labeling parameters. J Nucl Med. 1981; 22(11):981-7. View

Jinnouchi H, Sato Y, Cheng Q, Janifer C, Kutyna M, Cornelissen A . Thromboresistance and endothelial healing in polymer-coated versus polymer-free drug-eluting stents: Implications for short-term dual anti-platelet therapy. Int J Cardiol. 2020; 327:52-57. DOI: 10.1016/j.ijcard.2020.11.030. View

Chen M, Zamora P, Som P, Pena L, Osaki S . Cell attachment and biocompatibility of polytetrafluoroethylene (PTFE) treated with glow-discharge plasma of mixed ammonia and oxygen. J Biomater Sci Polym Ed. 2003; 14(9):917-35. DOI: 10.1163/156856203322381410. View

Urban P, Meredith I, Abizaid A, Pocock S, Carrie D, Naber C . Polymer-free Drug-Coated Coronary Stents in Patients at High Bleeding Risk. N Engl J Med. 2015; 373(21):2038-47. DOI: 10.1056/NEJMoa1503943. View

Taniwaki M, Radu M, Zaugg S, Amabile N, Garcia-Garcia H, Yamaji K . Mechanisms of Very Late Drug-Eluting Stent Thrombosis Assessed by Optical Coherence Tomography. Circulation. 2016; 133(7):650-60. DOI: 10.1161/CIRCULATIONAHA.115.019071. View

Seeger J, Ingegno M, Bigatan E, Klingman N, Amery D, Widenhouse C . Hydrophilic surface modification of metallic endoluminal stents. J Vasc Surg. 1995; 22(3):327-35; discussion 335-6. DOI: 10.1016/s0741-5214(95)70148-6. View

Tepe G, Wendel H, Khorchidi S, Schmehl J, Wiskirchen J, Pusich B . Thrombogenicity of various endovascular stent types: an in vitro evaluation. J Vasc Interv Radiol. 2002; 13(10):1029-35. DOI: 10.1016/s1051-0443(07)61868-5. View

Bowles D, Graier W, Sturek M . Hydrogen peroxide activates Na(+)-dependent Ca(2+) influx in coronary endothelial cells. Biochem Biophys Res Commun. 2001; 287(5):1134-9. DOI: 10.1006/bbrc.2001.5714. View

10.

Nakazawa G . Stent thrombosis of drug eluting stent: pathological perspective. J Cardiol. 2011; 58(2):84-91. DOI: 10.1016/j.jjcc.2011.07.004. View

11.

Capodanno D, Bhatt D, Gibson C, James S, Kimura T, Mehran R . Bleeding avoidance strategies in percutaneous coronary intervention. Nat Rev Cardiol. 2021; 19(2):117-132. DOI: 10.1038/s41569-021-00598-1. View

12.

Farb A, Burke A, Kolodgie F, Virmani R . Pathological mechanisms of fatal late coronary stent thrombosis in humans. Circulation. 2003; 108(14):1701-6. DOI: 10.1161/01.CIR.0000091115.05480.B0. View

13.

Curcio A, Torella D, Indolfi C . Mechanisms of smooth muscle cell proliferation and endothelial regeneration after vascular injury and stenting: approach to therapy. Circ J. 2011; 75(6):1287-96. DOI: 10.1253/circj.cj-11-0366. View

14.

Kotze H, Heyns A, Lotter M, Pieters H, Roodt J, Sweetlove M . Comparison of oxine and tropolone methods for labeling human platelets with indium-111. J Nucl Med. 1991; 32(1):62-6. View

15.

Rao J, Bei H, Yang Y, Liu Y, Lin H, Zhao X . Nitric Oxide-Producing Cardiovascular Stent Coatings for Prevention of Thrombosis and Restenosis. Front Bioeng Biotechnol. 2020; 8:578. PMC: 7326943. DOI: 10.3389/fbioe.2020.00578. View

16.

Milleret V, Ziogas A, Buzzi S, Heuberger R, Zucker A, Ehrbar M . Effect of oxide layer modification of CoCr stent alloys on blood activation and endothelial behavior. J Biomed Mater Res B Appl Biomater. 2014; 103(3):629-40. DOI: 10.1002/jbm.b.33232. View

17.

Sun D, Zheng Y, Yin T, Tang C, Yu Q, Wang G . Coronary drug-eluting stents: from design optimization to newer strategies. J Biomed Mater Res A. 2013; 102(5):1625-40. DOI: 10.1002/jbm.a.34806. View

18.

Varenne O, Cook S, Sideris G, Kedev S, Cuisset T, Carrie D . Drug-eluting stents in elderly patients with coronary artery disease (SENIOR): a randomised single-blind trial. Lancet. 2017; 391(10115):41-50. DOI: 10.1016/S0140-6736(17)32713-7. View

19.

Phan T, Jones J, Chen M, Bowles D, Fay W, Yu Q . A Biocompatibility Study of Plasma Nanocoatings onto Cobalt Chromium L605 Alloy for Cardiovascular Stent Applications. Materials (Basel). 2022; 15(17). PMC: 9457435. DOI: 10.3390/ma15175968. View

20.

Lyu N, Du Z, Qiu H, Gao P, Yao Q, Xiong K . Mimicking the Nitric Oxide-Releasing and Glycocalyx Functions of Endothelium on Vascular Stent Surfaces. Adv Sci (Weinh). 2020; 7(21):2002330. PMC: 7610264. DOI: 10.1002/advs.202002330. View