Alteration of Extracellular Matrix Proteins in Atrophic Periodontal Ligament of Hypofunctional Rat Molars

Overview

Journal BDJ Open

Specialty Dentistry

Date 2023 Jul 18

PMID 37463885

Authors

Daneeya Na Nan

Vincent Everts

Joao N Ferreira

Vorapat Trachoo

Thanaphum Osathanon

Nuttha Klincumhom

Prasit Pavasant

Affiliations

Soon will be listed here.

Abstract

Objectives: The aim of this study was to investigate the effect of mechanical force on possible dynamic changes of the matrix proteins deposition in the PDL upon in vitro mechanical and in vivo occlusal forces in a rat model with hypofunctional conditions.

Materials And Methods: Intermittent compressive force (ICF) and shear force (SF) were applied to human periodontal ligament stem cells (PDLSCs). Protein expression of collagen I and POSTN was analyzed by western blot technique. To establish an in vivo model, rat maxillary molars were extracted to facilitate hypofunction of the periodontal ligament (PDL) tissue of the opposing mandibular molar. The mandibles were collected after 4-, 8-, and 12-weeks post-extraction and used for micro-CT and immunohistochemical analysis.

Results: ICF and SF increased the synthesis of POSTN by human PDLSCs. Histological changes in the hypofunctional teeth revealed a narrowing of the PDL space, along with a decreased amount of collagen I, POSTN, and laminin in perivascular structures compared to the functional contralateral molars.

Conclusion: Our results revealed that loss of occlusal force disrupts deposition of some major matrix proteins in the PDL, underscoring the relevance of mechanical forces in maintaining periodontal tissue homeostasis by modulating ECM composition.

Citing Articles

Mechanical force modulates inflammation and immunomodulation in periodontal ligament cells.

Chansaenroj J, Suwittayarak R, Egusa H, Samaranayake L, Osathanon T Med Rev (2021). 2024; 4(6):544-548.

PMID: 39664083 PMC: 11629306. DOI: 10.1515/mr-2024-0034.

Effects of Improper Mechanical Force on the Production of Sonic Hedgehog, RANKL, and IL-6 in Human Periodontal Ligament Cells In Vitro.

Yamashita E, Negishi S, Kikuta J, Shimizu M, Senpuku H Dent J (Basel). 2024; 12(4).

PMID: 38668020 PMC: 11049549. DOI: 10.3390/dj12040108.

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