Leaf Alters Oxidative Stress, Protein Homeostasis and Ubiquitin-Proteasome Pathways in Fatty Acid-Induced Cell Line

Overview

Journal Food Technol Biotechnol

Specialty Biotechnology

Date 2023 Jul 17

PMID 37457905

Authors

Noor Nazirahanie Abrahim

Norhaniza Aminudin

Puteri Shafinaz Abdul-Rahman

Affiliations

Soon will be listed here.

Abstract

Research Background: (mistletoe fig) is a shrub well known among locals in Malaysia primarily for its treatment of toothaches, colds and wounds. The aim of this study is to determine the potential of leaves, sourced from three different varieties of , to exhibit antioxidant activity, a reduction of lipid concentration, and protein expression in steatosis-induced liver cell lines.

Experimental Approach: The leaves of three , namely var. , var. and var. , were subjected to water extraction. The resulting crude extracts were fractionated using water and ethyl acetate. Palmitic acid was used to induce lipid accumulation (steatosis) in human liver (WRL68) cells, before all the samples were tested for their lipid-reducing activity. Several proteomic approaches were incorporated. The changes in protein expression were determined using 2-dimensional gel electrophoresis separation, whereas identification of our protein spots of interest was carried out matrix-assisted laser desorption/ionization time-of-flight.

Results And Conclusions: var. alone demonstrated the ability to reduce lipids at the highest tested concentration (200 µg/mL) and was, therefore, used for subsequent experiments. Treatment with var. was found to restore redox status by increasing superoxide dismutase and glutathione peroxidase amounts and decreasing malondialdehyde formation. Six proteins were successfully identified; these were heat shock protein beta-1 (HSPB1), proteasome subunit alpha type 1 (PSMA1), glutathione S-transferase omega 1 (GSTO1), peroxiredoxin-1 (PRDX1), histone H2B (HIST1H2BD) and ubiquitin c-terminal hydrolase L3 (UCHL3). Through bioinformatics analysis, it was found that these proteins were significantly involved in specific pathways such as oxidative stress (PRDX1 and GSTO1), protein homeostasis (HSPB1) and degradation (UCHL3 and PSMA1).

Novelty And Scientific Contribution: pretreatment was shown to reduce lipid accumulation, thus improving the redox status and protein homeostasis. This suggests the role of as a preventive mechanism in non-alcohol fatty liver disease.

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