Investigating the Chemical Pathway to the Formation of a Single Biofilm Using Infrared Spectroscopy

Overview

Journal Biofilm

Date 2023 Jul 14

PMID 37449091

Authors

Amy R Crisp

Bryn Short

Laurence Rowan

Gordon Ramage

Ihtesham U R Rehman

Robert D Short

Craig Williams

Affiliations

Soon will be listed here.

Abstract

Diagnosing biofilm infections has remained a constant challenge for the last 50 years. Existing diagnostic methods struggle to identify the biofilm phenotype. Moreover, most methods of biofilm analysis destroy the biofilm making the resultant data interpretation difficult. In this study we introduce Fourier Transform Infra-Red (FTIR) spectroscopy as a label-free, non-destructive approach to monitoring biofilm progression. We have utilised FTIR in a novel application to evaluate the chemical composition of bacterial biofilms without disrupting the biofilm architecture. (RP62A) was grown onto calcium fluoride slides for periods of 30 min-96 h, before semi-drying samples for analysis. We report the discovery of a chemical marker to distinguish between planktonic and biofilm samples. The appearance of new proteins in biofilm samples of varying maturity is exemplified in the spectroscopic data, highlighting the potential of FTIR for identifying the presence and developmental stage of a single biofilm.

Citing Articles

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References

Alvarez-Ordonez A, Mouwen D, Lopez M, Prieto M . Fourier transform infrared spectroscopy as a tool to characterize molecular composition and stress response in foodborne pathogenic bacteria. J Microbiol Methods. 2011; 84(3):369-78. DOI: 10.1016/j.mimet.2011.01.009. View

Petibois C, Desbat B . Clinical application of FTIR imaging: new reasons for hope. Trends Biotechnol. 2010; 28(10):495-500. DOI: 10.1016/j.tibtech.2010.07.003. View

de la Fuente-Nunez C, Reffuveille F, Fernandez L, Hancock R . Bacterial biofilm development as a multicellular adaptation: antibiotic resistance and new therapeutic strategies. Curr Opin Microbiol. 2013; 16(5):580-9. DOI: 10.1016/j.mib.2013.06.013. View

Rabin N, Zheng Y, Opoku-Temeng C, Du Y, Bonsu E, Sintim H . Biofilm formation mechanisms and targets for developing antibiofilm agents. Future Med Chem. 2015; 7(4):493-512. DOI: 10.4155/fmc.15.6. View

Lindsay D, Von Holy A . Bacterial biofilms within the clinical setting: what healthcare professionals should know. J Hosp Infect. 2006; 64(4):313-25. DOI: 10.1016/j.jhin.2006.06.028. View

Quiles F, Polyakov P, Humbert F, Francius G . Production of extracellular glycogen by Pseudomonas fluorescens: spectroscopic evidence and conformational analysis by biomolecular recognition. Biomacromolecules. 2012; 13(7):2118-27. DOI: 10.1021/bm300497c. View

Gupta S, Andersen C, Black J, de Leon J, Fife C, Lantis Ii J . Management of Chronic Wounds: Diagnosis, Preparation, Treatment, and Follow-up. Wounds. 2017; 29(9):S19-S36. View

Berthomieu C, Hienerwadel R . Fourier transform infrared (FTIR) spectroscopy. Photosynth Res. 2009; 101(2-3):157-70. DOI: 10.1007/s11120-009-9439-x. View

Nivens D, Ohman D, Williams J, Franklin M . Role of alginate and its O acetylation in formation of Pseudomonas aeruginosa microcolonies and biofilms. J Bacteriol. 2001; 183(3):1047-57. PMC: 94973. DOI: 10.1128/JB.183.3.1047-1057.2001. View

10.

Lawrence J, Korber D, Hoyle B, Costerton J, Caldwell D . Optical sectioning of microbial biofilms. J Bacteriol. 1991; 173(20):6558-67. PMC: 208993. DOI: 10.1128/jb.173.20.6558-6567.1991. View

11.

Srivastava S, Bhargava A . Biofilms and human health. Biotechnol Lett. 2015; 38(1):1-22. DOI: 10.1007/s10529-015-1960-8. View

12.

Gorman S, Mawhinney W, Adair C, Issouckis M . Confocal laser scanning microscopy of peritoneal catheter surfaces. J Med Microbiol. 1993; 38(6):411-7. DOI: 10.1099/00222615-38-6-411. View

13.

Skogman M, Vuorela P, Fallarero A . Combining biofilm matrix measurements with biomass and viability assays in susceptibility assessments of antimicrobials against Staphylococcus aureus biofilms. J Antibiot (Tokyo). 2012; 65(9):453-9. DOI: 10.1038/ja.2012.49. View

14.

Wenning M, Scherer S . Identification of microorganisms by FTIR spectroscopy: perspectives and limitations of the method. Appl Microbiol Biotechnol. 2013; 97(16):7111-20. DOI: 10.1007/s00253-013-5087-3. View

15.

Hassan A, Usman J, Kaleem F, Omair M, Khalid A, Iqbal M . Evaluation of different detection methods of biofilm formation in the clinical isolates. Braz J Infect Dis. 2011; 15(4):305-11. View

16.

Hall-Stoodley L, Costerton J, Stoodley P . Bacterial biofilms: from the natural environment to infectious diseases. Nat Rev Microbiol. 2004; 2(2):95-108. DOI: 10.1038/nrmicro821. View

17.

Holman H, Miles R, Hao Z, Wozei E, Anderson L, Yang H . Real-time chemical imaging of bacterial activity in biofilms using open-channel microfluidics and synchrotron FTIR spectromicroscopy. Anal Chem. 2009; 81(20):8564-70. DOI: 10.1021/ac9015424. View

18.

Serra D, Bosch A, Russo D, Rodriguez M, Zorreguieta A, Schmitt J . Continuous nondestructive monitoring of Bordetella pertussis biofilms by Fourier transform infrared spectroscopy and other corroborative techniques. Anal Bioanal Chem. 2007; 387(5):1759-67. DOI: 10.1007/s00216-006-1079-9. View

19.

Severn M, Horswill A . Staphylococcus epidermidis and its dual lifestyle in skin health and infection. Nat Rev Microbiol. 2022; 21(2):97-111. PMC: 9903335. DOI: 10.1038/s41579-022-00780-3. View

20.

Solano C, Echeverz M, Lasa I . Biofilm dispersion and quorum sensing. Curr Opin Microbiol. 2014; 18:96-104. DOI: 10.1016/j.mib.2014.02.008. View