Histochemical Localization of Cholinesterases and Monoamines in the Central Heart of Sepia Officinalis L. (Cephalopoda)

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1986 Jan 1

PMID 3744906

Citations 3

Authors

G Kling

Affiliations

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Abstract

The central heart of the coleoid cephalopod, Sepia officinalis, was studied using acetylcholinesterase and fluorescence histochemistry. Using histo- and cytochemical reactions, acetylcholinesterase was localized in the axolemma and axoplasm of specific cardiac nerve fibres, as well as in the sarcolemma and within the sarcotubular system of the muscle cells. Butyrylcholinesterase exhibited a different distribution, being found only in the luminal trabecular muscle layer. Glyoxylic-acid-induced fluorescence indicated the presence of catecholamines (emission maximum, 470 nm) in cardiac nerve axons. These histochemical findings support the hypothesis that noradrenaline and/or dopamine and acetylcholine act antagonistically as natural transmitters. Fluorophores indicating the presence of serotonin were not observed. The present results are discussed in the light of previous pharmacological findings.

Citing Articles

Distribution and function of biogenic amines in the heart of Nautilus pompilius L. (Cephalopoda, Tetrabranchiata).

Springer J, Ruth P, Beuerlein K, Palus S, Schipp R, Westermann B J Mol Histol. 2005; 36(5):345-53.

PMID: 16240167 DOI: 10.1007/s10735-005-9006-5.

Localization of putative neurotransmitters in the mantle and siphuncle of the mollusc Nautilus pompilius L. (Cephalopoda).

Westermann B, Beuerlein K, Hempelmann G, Schipp R Histochem J. 2003; 34(8-9):435-40.

PMID: 12814191 DOI: 10.1023/a:1023691606047.

Comparison of butyrylcholinesterase and acetylcholinesterase.

Chatonnet A, Lockridge O Biochem J. 1989; 260(3):625-34.

PMID: 2669736 PMC: 1138724. DOI: 10.1042/bj2600625.

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