Genome-Wide Identification of the PP2C Gene Family and Analyses with Their Expression Profiling in Response to Cold Stress in Wild Sugarcane

Overview

Journal Plants (Basel)

Date 2023 Jul 14

PMID 37446979

Authors

Xing Huang

Yongsheng Liang

Ronghua Zhang

Baoqing Zhang

Xiupeng Song

Junxian Liu

Manman Lu

Zhenqiang Qin

DeWei Li

Song Li

Yangrui Li

Affiliations

Soon will be listed here.

Abstract

Type 2C protein phosphatases (PP2Cs) represent a major group of protein phosphatases in plants, some of which have already been confirmed to play important roles in diverse plant processes. In this study, analyses of the phylogenetics, gene structure, protein domain, chromosome localization, and collinearity, as well as an identification of the expression profile, protein-protein interaction, and subcellular location, were carried out on the PP2C family in wild sugarcane (). The results showed that 145 PP2C proteins were classified into 13 clades. Phylogenetic analysis suggested that SsPP2Cs are evolutionarily closer to those of sorghum, and the number of SsPP2Cs is the highest. There were 124 pairs of genes expanding via segmental duplications. Half of the SsPP2C proteins were predicted to be localized in the chloroplast (73), with the next most common predicted localizations being in the cytoplasm (37) and nucleus (17). Analysis of the promoter revealed that s might be photosensitive, responsive to abiotic stresses, and hormone-stimulated. A total of 27 s showed cold-stress-induced expressions, and (Sspon.01G0007840-2D) and (Sspon.03G0002800-3D) were the potential hubs involved in ABA signal transduction. Our study presents a comprehensive analysis of the SsPP2C gene family, which can play a vital role in the further study of phosphatases in wild sugarcane. The results suggest that the PP2C family is evolutionarily conserved, and that it functions in various developmental processes in wild sugarcane.

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