Study on Extraction, Physicochemical Properties, and Bacterio-Static Activity of Polysaccharides from

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Jul 14

PMID 37446762

Authors

Nengbin Gao

Weijia Zhang

Dianjie Hu

Guo Lin

Jingxuan Wang

Feng Xue

Qian Wang

Hongfei Zhao

Xin Dou

Lihong Zhang

Affiliations

Soon will be listed here.

Abstract

We optimized an ultrasound-assisted extraction process of mycelium polysaccharides (PLPs) and studied their monosaccharide composition and bacteriostatic properties. Based on a single-factor experiment, a three-factor, three-level Box-Behnken design was used to optimize the ultrasound-assisted extraction process of PLP, using the yield of PLP as the index. The chemical composition and monosaccharide composition of PLP were determined by chemical analysis and HPLC analysis, respectively. Microscopic morphological analysis of the surface of PLP was performed via swept-surface electron microscopy. The bacteriostatic properties of PLP were determined using the spectrophotometric turbidimetric method. The results showed that the best extraction process of PLP with ultrasonic assistance achieved a result of 1:42 g/mL. In this method, the ultrasonic temperature was 60 °C, ultrasonic extraction was performed for 20 min, and the yield of PLP was 12.98%. The monosaccharide composition of PLP mainly contains glucose (Glc), mannose (Man), galactose (Gal), and glucuronic acid (GlcA). The intracellular polysaccharide of Phellinus igniarius Mycelia (PIP) is an irregular spherical accumulation, the surface is rough and not smooth, and the extracellular polysaccharide (PEP) is a crumbly accumulation. PIP has a stronger inhibitory ability for and and a slightly weaker inhibitory effect for ; the inhibitory effect of PEP on , , and is slightly inferior to that of PIP.

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