Transcriptomic Profiling Highlights the ABA Response Role of in

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Jul 14

PMID 37445818

Authors

Chi Zhang

Xiaoqing Yao

Yan Zhang

Shengbo Zhao

Jinghui Liu

Gang Wu

Xiaohong Yan

Junling Luo

Affiliations

Soon will be listed here.

Abstract

is the first identified SIP1 (6b Interacting Protein1) subfamily gene of the trihelix transcription factor family from We previously used a reverse genetic method to reveal its abiotic stress response function in endowing plants resistance to drought and salinity, as well as ABA (Abscisic acid). However, the molecular mechanisms of are unclear. In this study, the global transcriptome files of -overexpressing transgenic and wildtype seedlings under ABA treatment were constructed using RNA-seq. A total of 1823 and 5512 DEGs (Differentially Expressed Genes) were identified in OE vs. WT and OE_ABA vs. WT_ABA comparison groups, which included 751 and 2567 up-regulated DEGs, and 1072 and 2945 down-regulated DEGs, separately. The impact of overexpressed on plants was amplified by ABA, indicating was an ABA-conditioned responsive gene. More interestingly, we found the reasons for increasing plants' insensitivity to ABA were not by regulating ABA synthesis and catabolism, but by manipulating ABA transportation, ABA signal perception and transduction, inositol phosphate metabolism, as well as endomembrane trafficking, indirectly suggesting this gene may play roles upstream of the core ABA response pathway. Our results provided new insights into improving the knowledge about the function of and the ABA signaling mechanism in

Citing Articles

Positively Regulates Drought Tolerance and Enhanced Stomatal Response through the MAPK Signaling Pathway in Soybean.

Zhao Y, Wang S, Ma X, He Y, Zhou J, Jiao S Int J Mol Sci. 2024; 25(13).

PMID: 39000082 PMC: 11241039. DOI: 10.3390/ijms25136972.

References

Romeis T, Piedras P, Zhang S, Klessig D, Hirt H, Jones J . Rapid Avr9- and Cf-9 -dependent activation of MAP kinases in tobacco cell cultures and leaves: convergence of resistance gene, elicitor, wound, and salicylate responses. Plant Cell. 1999; 11(2):273-87. PMC: 144176. DOI: 10.1105/tpc.11.2.273. View

Jahn R, Scheller R . SNAREs--engines for membrane fusion. Nat Rev Mol Cell Biol. 2006; 7(9):631-43. DOI: 10.1038/nrm2002. View

Okamoto M, Tanaka Y, Abrams S, Kamiya Y, Seki M, Nambara E . High humidity induces abscisic acid 8'-hydroxylase in stomata and vasculature to regulate local and systemic abscisic acid responses in Arabidopsis. Plant Physiol. 2008; 149(2):825-34. PMC: 2633821. DOI: 10.1104/pp.108.130823. View

Wang X, Xu M, Gao C, Zeng Y, Cui Y, Shen W . The roles of endomembrane trafficking in plant abiotic stress responses. J Integr Plant Biol. 2019; 62(1):55-69. DOI: 10.1111/jipb.12895. View

Kaplan-Levy R, Brewer P, Quon T, Smyth D . The trihelix family of transcription factors--light, stress and development. Trends Plant Sci. 2012; 17(3):163-71. DOI: 10.1016/j.tplants.2011.12.002. View

Kwon H, Cho D, Lee H, Nam M, Kwon C, Yun H . CCOAOMT1, a candidate cargo secreted via VAMP721/722 secretory vesicles in Arabidopsis. Biochem Biophys Res Commun. 2020; 524(4):977-982. DOI: 10.1016/j.bbrc.2020.02.029. View

Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden T . Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics. 2012; 13:134. PMC: 3412702. DOI: 10.1186/1471-2105-13-134. View

Shang Y, Yan L, Liu Z, Cao Z, Mei C, Xin Q . The Mg-chelatase H subunit of Arabidopsis antagonizes a group of WRKY transcription repressors to relieve ABA-responsive genes of inhibition. Plant Cell. 2010; 22(6):1909-35. PMC: 2910980. DOI: 10.1105/tpc.110.073874. View

Jia Q, Kong D, Li Q, Sun S, Song J, Zhu Y . The Function of Inositol Phosphatases in Plant Tolerance to Abiotic Stress. Int J Mol Sci. 2019; 20(16). PMC: 6719168. DOI: 10.3390/ijms20163999. View

10.

Luo J, Tang S, Mei F, Peng X, Li J, Li X . , a Trihelix Family Gene, Mediates Abiotic Stress Tolerance and ABA Signaling in . Front Plant Sci. 2017; 8:44. PMC: 5266734. DOI: 10.3389/fpls.2017.00044. View

11.

Yoshida T, Christmann A, Yamaguchi-Shinozaki K, Grill E, Fernie A . Revisiting the Basal Role of ABA - Roles Outside of Stress. Trends Plant Sci. 2019; 24(7):625-635. DOI: 10.1016/j.tplants.2019.04.008. View

12.

Rodriguez P, Benning G, Grill E . ABI2, a second protein phosphatase 2C involved in abscisic acid signal transduction in Arabidopsis. FEBS Lett. 1998; 421(3):185-90. DOI: 10.1016/s0014-5793(97)01558-5. View

13.

Mitula F, Tajdel M, Ciesla A, Kasprowicz-Maluski A, Kulik A, Babula-Skowronska D . Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015; 56(12):2351-67. PMC: 4675898. DOI: 10.1093/pcp/pcv146. View

14.

Geva Y, Schuldiner M . The back and forth of cargo exit from the endoplasmic reticulum. Curr Biol. 2014; 24(3):R130-6. DOI: 10.1016/j.cub.2013.12.008. View

15.

Babula-Skowronska D, Ludwikow A, Ciesla A, Olejnik A, Cegielska-Taras T, Bartkowiak-Broda I . Involvement of genes encoding ABI1 protein phosphatases in the response of Brassica napus L. to drought stress. Plant Mol Biol. 2015; 88(4-5):445-57. PMC: 4486095. DOI: 10.1007/s11103-015-0334-x. View

16.

Sharipova G, Veselov D, Kudoyarova G, Fricke W, Dodd I, Katsuhara M . Exogenous application of abscisic acid (ABA) increases root and cell hydraulic conductivity and abundance of some aquaporin isoforms in the ABA-deficient barley mutant Az34. Ann Bot. 2016; 118(4):777-785. PMC: 5055630. DOI: 10.1093/aob/mcw117. View

17.

Wang W, Wu P, Liu T, Ren H, Li Y, Hou X . Genome-wide Analysis and Expression Divergence of the Trihelix family in Brassica Rapa: Insight into the Evolutionary Patterns in Plants. Sci Rep. 2017; 7(1):6463. PMC: 5526864. DOI: 10.1038/s41598-017-06935-0. View

18.

Yi C, Park S, Yun H, Kwon C . Vesicle-associated membrane proteins 721 and 722 are required for unimpeded growth of Arabidopsis under ABA application. J Plant Physiol. 2012; 170(5):529-33. DOI: 10.1016/j.jplph.2012.11.001. View

19.

Jones P, Binns D, Chang H, Fraser M, Li W, McAnulla C . InterProScan 5: genome-scale protein function classification. Bioinformatics. 2014; 30(9):1236-40. PMC: 3998142. DOI: 10.1093/bioinformatics/btu031. View

20.

Gillaspy G . The cellular language of myo-inositol signaling. New Phytol. 2011; 192(4):823-839. DOI: 10.1111/j.1469-8137.2011.03939.x. View