Is a NAC Transcription Factor of Chinese Fir in Response to Phosphate Starvation

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Jul 14

PMID 37445664

Authors

Yuxuan Zhao

Shuotian Huang

Lihui Wei

Meng Li

Tingting Cai

Xiangqing Ma

Peng Shuai

Affiliations

Soon will be listed here.

Abstract

Phosphate (Pi) deficiency is one of the most limiting factors for Chinese fir growth and production. Moreover, continuous cultivation of Chinese fir for multiple generations led to the reduction of soil nutrients, which hindered the yield of Chinese fir in southern China. Although NAC (NAM, ATAF, and CUC) transcription factors (TFs) play critical roles in plant development and abiotic stress resistance, it is still unclear how they regulate the response of Chinese fir to phosphate (Pi) starvation. Based on Pi-deficient transcriptome data of Chinses fir root, we identified a NAC transcription factor with increased expression under Pi deficiency, which was obtained by PCR and named . RT-qPCR confirmed that the expression of in the root of Chinese fir was induced by phosphate deficiency and showed a dynamic change with time. It was positively regulated by ABA and negatively regulated by JA, and was highly expressed in the roots and leaves of Chinese fir. Transcriptional activation assay confirmed that was a transcriptional activator. The promoter of was obtained by genome walking, which was predicted to contain a large number of stress, hormone, and growth-related cis-elements. Tobacco infection was used to verify the activity of the promoter, and the core promoter was located between -1519 bp and -589 bp. We identified 18 proteins bound to the promoter and 5 ClNAC100 interacting proteins by yeast one-hybrid and yeast two-hybrid, respectively. We speculated that AHL and TIFY family transcription factors, calmodulin, and E3 ubiquitin ligase in these proteins might be important phosphorus-related proteins. These results provide a basis for the further study of the regulatory mechanism and pathways of under Pi starvation.

References

Xie C, Ding Z . NAC1 Maintains Root Meristem Activity by Repressing the Transcription of in . Int J Mol Sci. 2022; 23(20). PMC: 9603599. DOI: 10.3390/ijms232012258. View

Ham B, Chen J, Yan Y, Lucas W . Insights into plant phosphate sensing and signaling. Curr Opin Biotechnol. 2017; 49:1-9. DOI: 10.1016/j.copbio.2017.07.005. View

Sun Y, Wu Q, Xie Z, Huang J . Transcription factor OsNAC016 negatively regulates phosphate-starvation response in rice. Plant Sci. 2023; 329:111618. DOI: 10.1016/j.plantsci.2023.111618. View

Chiou T, Lin S . Signaling network in sensing phosphate availability in plants. Annu Rev Plant Biol. 2011; 62:185-206. DOI: 10.1146/annurev-arplant-042110-103849. View

Zhu X, Wang P, Bai Z, Herde M, Ma Y, Li N . Calmodulin-like protein CML24 interacts with CAMTA2 and WRKY46 to regulate ALMT1-dependent Al resistance in Arabidopsis thaliana. New Phytol. 2021; 233(6):2471-2487. DOI: 10.1111/nph.17812. View

Shen S, Zhang Q, Shi Y, Sun Z, Zhang Q, Hou S . Genome-Wide Analysis of the NAC Domain Transcription Factor Gene Family in . Genes (Basel). 2020; 11(1). PMC: 7017368. DOI: 10.3390/genes11010035. View

Tran L, Nakashima K, Sakuma Y, Simpson S, Fujita Y, Maruyama K . Isolation and functional analysis of Arabidopsis stress-inducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1 promoter. Plant Cell. 2004; 16(9):2481-98. PMC: 520947. DOI: 10.1105/tpc.104.022699. View

Zhang J, Zhou X, Xu Y, Yao M, Xie F, Gai J . Soybean SPX1 is an important component of the response to phosphate deficiency for phosphorus homeostasis. Plant Sci. 2016; 248:82-91. DOI: 10.1016/j.plantsci.2016.04.010. View

Hafiz Baillo E, Kimotho R, Zhang Z, Xu P . Transcription Factors Associated with Abiotic and Biotic Stress Tolerance and Their Potential for Crops Improvement. Genes (Basel). 2019; 10(10). PMC: 6827364. DOI: 10.3390/genes10100771. View

10.

Williamson L, Ribrioux S, Fitter A, Leyser H . Phosphate availability regulates root system architecture in Arabidopsis. Plant Physiol. 2001; 126(2):875-82. PMC: 111176. DOI: 10.1104/pp.126.2.875. View

11.

Grunewald W, Vanholme B, Pauwels L, Plovie E, Inze D, Gheysen G . Expression of the Arabidopsis jasmonate signalling repressor JAZ1/TIFY10A is stimulated by auxin. EMBO Rep. 2009; 10(8):923-8. PMC: 2726675. DOI: 10.1038/embor.2009.103. View

12.

Ramaiah M, Jain A, Yugandhar P, Raghothama K . ATL8, a RING E3 ligase, modulates root growth and phosphate homeostasis in Arabidopsis. Plant Physiol Biochem. 2022; 179:90-99. DOI: 10.1016/j.plaphy.2022.03.019. View

13.

Ernst H, Olsen A, Larsen S, Lo Leggio L . Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors. EMBO Rep. 2004; 5(3):297-303. PMC: 1299004. DOI: 10.1038/sj.embor.7400093. View

14.

Ye Q, Wang H, Su T, Wu W, Chen Y . The Ubiquitin E3 Ligase PRU1 Regulates WRKY6 Degradation to Modulate Phosphate Homeostasis in Response to Low-Pi Stress in Arabidopsis. Plant Cell. 2018; 30(5):1062-1076. PMC: 6002188. DOI: 10.1105/tpc.17.00845. View

15.

Nilsson L, Muller R, Nielsen T . Dissecting the plant transcriptome and the regulatory responses to phosphate deprivation. Physiol Plant. 2010; 139(2):129-43. DOI: 10.1111/j.1399-3054.2010.01356.x. View

16.

Singh P, Mukhopadhyay K . Comprehensive molecular dissection of TIFY Transcription factors reveal their dynamic responses to biotic and abiotic stress in wheat (Triticum aestivum L.). Sci Rep. 2021; 11(1):9739. PMC: 8102568. DOI: 10.1038/s41598-021-87722-w. View

17.

Baek D, Kim M, Chun H, Kang S, Park H, Shin G . Regulation of miR399f transcription by AtMYB2 affects phosphate starvation responses in Arabidopsis. Plant Physiol. 2012; 161(1):362-73. PMC: 3532267. DOI: 10.1104/pp.112.205922. View

18.

Wong M, Bhaskara G, Wen T, Lin W, Nguyen T, Chong G . Phosphoproteomics of Highly ABA-Induced1 identifies AT-Hook-Like10 phosphorylation required for stress growth regulation. Proc Natl Acad Sci U S A. 2019; 116(6):2354-2363. PMC: 6369736. DOI: 10.1073/pnas.1819971116. View

19.

Srivastava S, Upadhyay M, Srivastava A, Abdelrahman M, Suprasanna P, Tran L . Cellular and Subcellular Phosphate Transport Machinery in Plants. Int J Mol Sci. 2018; 19(7). PMC: 6073359. DOI: 10.3390/ijms19071914. View

20.

Devaiah B, Madhuvanthi R, Karthikeyan A, Raghothama K . Phosphate starvation responses and gibberellic acid biosynthesis are regulated by the MYB62 transcription factor in Arabidopsis. Mol Plant. 2009; 2(1):43-58. PMC: 2639739. DOI: 10.1093/mp/ssn081. View