High Prevalence of Pantoea Spp. in Microbiota Associated with the Sorghum Plant Bug Stenotus Rubrovittatus (Heteroptera: Miridae)

Overview

Journal Microbes Environ

Specialty Environmental Health

Date 2023 Jul 12

PMID 37438113

Authors

Yuto Sato

Tatsuki Akao

Kazutaka Takeshita

Affiliations

Soon will be listed here.

Abstract

The sorghum plant bug, Stenotus rubrovittatus (order Heteroptera: family Miridae), is a notorious insect pest in Japan that causes pecky rice. In the present study, we sampled this insect pest in the northern part of Honshu Island in Japan and investigated its associated microbiota. The results obtained showed that Pantoea dominated the associated microbiota and was the sole genus detected in all samples. The dominant Pantoea were phylogenetically close to rice pathogens. The present results suggest that the sorghum plant bug needs to be regarded and controlled not only as a notorious insect pest, but also as a potential vector of rice pathogenic Pantoea spp.

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References

Stamatakis A, Hoover P, Rougemont J . A rapid bootstrap algorithm for the RAxML Web servers. Syst Biol. 2008; 57(5):758-71. DOI: 10.1080/10635150802429642. View

Luo J, Cheng Y, Guo L, Wang A, Lu M, Xu L . Variation of gut microbiota caused by an imbalance diet is detrimental to bugs' survival. Sci Total Environ. 2021; 771:144880. DOI: 10.1016/j.scitotenv.2020.144880. View

Florez L, Scherlach K, Gaube P, Ross C, Sitte E, Hermes C . Antibiotic-producing symbionts dynamically transition between plant pathogenicity and insect-defensive mutualism. Nat Commun. 2017; 8:15172. PMC: 5414355. DOI: 10.1038/ncomms15172. View

Stoddard S, Smith B, Hein R, Roller B, Schmidt T . rrnDB: improved tools for interpreting rRNA gene abundance in bacteria and archaea and a new foundation for future development. Nucleic Acids Res. 2014; 43(Database issue):D593-8. PMC: 4383981. DOI: 10.1093/nar/gku1201. View

Xue H, Zhu X, Wang L, Zhang K, Li D, Ji J . Gut Bacterial Diversity in Different Life Cycle Stages of (Hemiptera: Miridae). Front Microbiol. 2021; 12:670383. PMC: 8208491. DOI: 10.3389/fmicb.2021.670383. View

Dally M, Lalzar M, Belausov E, Gottlieb Y, Coll M, Zchori-Fein E . Cellular Localization of Two Symbionts in the Digestive System and within the Ovaries of the Mirid Bug, . Insects. 2020; 11(8). PMC: 7469188. DOI: 10.3390/insects11080530. View

Stamatakis A . RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics. 2014; 30(9):1312-3. PMC: 3998144. DOI: 10.1093/bioinformatics/btu033. View

Wang Q, Garrity G, Tiedje J, Cole J . Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol. 2007; 73(16):5261-7. PMC: 1950982. DOI: 10.1128/AEM.00062-07. View

Douglas A . Multiorganismal insects: diversity and function of resident microorganisms. Annu Rev Entomol. 2014; 60:17-34. PMC: 4465791. DOI: 10.1146/annurev-ento-010814-020822. View

10.

Hosokawa T, Kikuchi Y, Shimada M, Fukatsu T . Obligate symbiont involved in pest status of host insect. Proc Biol Sci. 2007; 274(1621):1979-84. PMC: 2275188. DOI: 10.1098/rspb.2007.0620. View

11.

Duron O, Noel V . A wide diversity of Pantoea lineages are engaged in mutualistic symbiosis and cospeciation processes with stinkbugs. Environ Microbiol Rep. 2016; 8(5):715-727. DOI: 10.1111/1758-2229.12432. View

12.

Itoh H, Tago K, Hayatsu M, Kikuchi Y . Detoxifying symbiosis: microbe-mediated detoxification of phytotoxins and pesticides in insects. Nat Prod Rep. 2018; 35(5):434-454. DOI: 10.1039/c7np00051k. View

13.

Rognes T, Flouri T, Nichols B, Quince C, Mahe F . VSEARCH: a versatile open source tool for metagenomics. PeerJ. 2016; 4:e2584. PMC: 5075697. DOI: 10.7717/peerj.2584. View

14.

Lee H, Hong J, Kim S . First Report of Leaf Blight Caused by Pantoea agglomerans on Rice in Korea. Plant Dis. 2019; 94(11):1372. DOI: 10.1094/PDIS-05-10-0374. View

15.

Hosokawa T, Ishii Y, Nikoh N, Fujie M, Satoh N, Fukatsu T . Obligate bacterial mutualists evolving from environmental bacteria in natural insect populations. Nat Microbiol. 2016; 1:15011. DOI: 10.1038/nmicrobiol.2015.11. View

16.

Sato Y, Jang S, Takeshita K, Itoh H, Koike H, Tago K . Insecticide resistance by a host-symbiont reciprocal detoxification. Nat Commun. 2021; 12(1):6432. PMC: 8571283. DOI: 10.1038/s41467-021-26649-2. View

17.

Barash I, Manulis-Sasson S . Virulence mechanisms and host specificity of gall-forming Pantoea agglomerans. Trends Microbiol. 2007; 15(12):538-45. DOI: 10.1016/j.tim.2007.10.009. View

18.

Coolen S, Magda R, Welte C . The secret life of insect-associated microbes and how they shape insect-plant interactions. FEMS Microbiol Ecol. 2022; 98(9). PMC: 9409087. DOI: 10.1093/femsec/fiac083. View

19.

Chang K, MUSGRAVE A . Ultrastructure of rickettsia-like microorganisms in the midgut of a plant bug, Stenotus binotatus Jak. (Heteroptera: Miridae). Can J Microbiol. 1970; 16(7):621-2. DOI: 10.1139/m70-104. View

20.

Lv L, Luo J, Ahmed T, Zaki H, Tian Y, Shahid M . Beneficial Effect and Potential Risk of on Rice Production. Plants (Basel). 2022; 11(19). PMC: 9570785. DOI: 10.3390/plants11192608. View