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» Articles » PMID: 374369

Rapid Methods for Generalized Transduction of Salmonella Typhimurium Mutants

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1979 Apr 1
PMID 374369
Citations 5
Authors
S A Rosenfeld
J Brenchley
Affiliations
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Abstract

A procedure has been developed that allows the propagation of generalized transducing phage directly on cells growing on solid media. After the donor cells are killed with chloroform, the phage can be transferred directly to recipient cells and transductants can be selected.

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References
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Brenchley J . Effect of methionine sulfoximine and methionine sulfone on glutamate synthesis in Klebsiella aerogenes. J Bacteriol. 1973; 114(2):666-73. PMC: 251824. DOI: 10.1128/jb.114.2.666-673.1973. View
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McIntire S . Transduction with integration-defective mutants of Salmonella typhimurium bacteriophage KB1. J Bacteriol. 1974; 117(2):907-8. PMC: 285589. DOI: 10.1128/jb.117.2.907-908.1974. View
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SCHMIEGER H . Phage P22-mutants with increased or decreased transduction abilities. Mol Gen Genet. 1972; 119(1):75-88. DOI: 10.1007/BF00270447. View