A Transcriptional Regulator Gar Regulates the Growth and Virulence of Xanthomonas Oryzae Pv. Oryzae

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2023 Jul 12

PMID 37436661

Authors

Huihui Wang

Fan Chen

Guiyu Tang

Wenli Ke

Shasha Wang

Dehong Zheng

Lifang Ruan

Affiliations

Soon will be listed here.

Abstract

Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of bacterial blight, one of the most devastating diseases of rice. Pathogenic bacteria possess numerous transcriptional regulators to participate in the regulation of cellular processes. Here, we identified a transcriptional regulator Gar (PXO_RS11965) that is involved in regulating the growth and virulence of Xoo. Notably, the knockout of gar in Xoo enhanced bacterial virulence to the host rice. RNA-sequencing analysis and quantitative β-glucuronidase (GUS) assay indicated that Gar positively regulates the expression of a σ factor rpoN2. Further experiments confirmed that overexpression of rpoN2 restored the phenotypic changes caused by gar deletion. Our research revealed that Gar influences bacterial growth and virulence by positively regulating the expression of rpoN2.

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