Evaluating the Typing Power of Six Isoenzymatic Systems for Differentiation of Clinical and Standard Isolates of Candida Species

Overview

Journal Adv Biomed Res

Specialty Biomedical Engineering

Date 2023 Jul 12

PMID 37434931

Authors

Gholamreza Hatam

Hamid Morovati

Marzieh Alikhani

Amir Rahimi

Keyvan Pakshir

Sara Amini

Kamiar Zomorodian

Affiliations

Soon will be listed here.

Abstract

Background: Due to the increasing prevalence of candidiasis, early detection of the causative agents may pave the way for the management of this infection. The present study aimed to assess the discriminative power of the six isoenzymatic systems for differentiating the species.

Materials And Methods: Sixteen standard and strains and 30 fluconazole-sensitive and fluconazole-resistant clinical strains of were analyzed using a Multilocus Enzyme Electrophoresis (MLEE) method, including six enzymatic systems consisting of malate dehydrogenase (MDH), phosphoglucomutase (PGM), glucose-phosphate isomerase (GPI), glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGD), and malic enzyme (ME).

Results: Among the six enzymatic systems, ME showed no diagnostic activity, whereas MDH provided the best species-specific pattern for species discrimination. In addition, the MDH and G6PD systems provided a discriminatory pattern for differentiating from isolates. The same isoenzymatic activity was detected in all 36 standard and clinical isolates. Moreover, the results showed no correlation between the isoenzymatic profiles and drug resistance.

Conclusion: Among the investigated MLEE systems, MDH was able to differentiate between and . Although no association was detected between isoenzyme patterns and fluconazole resistance in this investigation, isoenzyme patterns are likely correlated with virulence factors between species and even within species. To answer these questions, additional studies should be done on more strains.

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