Lemon-derived Nanovesicles Achieve Antioxidant and Anti-inflammatory Effects Activating the AhR/Nrf2 Signaling Pathway

Overview

Journal iScience

Publisher Cell Press

Date 2023 Jul 10

PMID 37426343

Authors

Ornella Urzi

Marco Cafora

Nima Rabienezhad Ganji

Vincenza Tinnirello

Roberta Gasparro

Samuele Raccosta

Mauro Manno

Anna Maria Corsale

Alice Conigliaro

Anna Pistocchi

Stefania Raimondo

Riccardo Alessandro

Affiliations

Soon will be listed here.

Abstract

In the last years, extracellular vesicles (EVs) from different plant matrices have been isolated and gained the interest of the scientific community for their intriguing biological properties. In this study, we isolated and characterized nanovesicles from lemon juice (LNVs) and evaluated their antioxidant effects. We tested LNV antioxidant activity using human dermal fibroblasts that were pre-treated with LNVs for 24 h and then stimulated with hydrogen peroxide (HO) and UVB irradiation. We found that LNV pre-treatment reduced ROS levels in fibroblasts stimulated with HO and UVB. This reduction was associated with the activation of the AhR/Nrf2 signaling pathway, whose protein expression and nuclear localization was increased in fibroblasts treated with LNVs. By using zebrafish embryos as model, we confirmed the antioxidant effects of LNVs. We found that LNVs reduced ROS levels and neutrophil migration in zebrafish embryos stimulated with LPS.

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