High-Fat and High-Sucrose Diet Leads to Skeletal Muscle Loss and Bladder Dysfunction in Rat

Overview

Journal Res Rep Urol

Publisher Dove Medical Press

Specialty Urology

Date 2023 Jul 10

PMID 37425652

Authors

Naoki Wada

Noriyuki Abe

Kotona Miyauchi

Shogo Makino

Hidehiro Kakizaki

Affiliations

Soon will be listed here.

Abstract

Purpose: In this study, we investigated skeletal muscle loss and bladder dysfunction caused by high-fat/high-sucrose (HFS) diet.

Methods: Twelve-week-old Sprague-Dawley (SD) female rats were fed on normal (Group N) or HFS (Group HFS) diet for 12 weeks. We conducted urodynamic investigation and pharmacologic in vitro. In addition, we measured gastrocnemius and tibialis muscle weight and protein concentration. The hypoxia-inducible factor (HIF)-1α and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the bladder were assayed.

Results: The urodynamic investigations revealed the significantly shorter intercontraction intervals and lower maximal voiding pressure in Group HFS than in Group N. Furthermore, the absolute and relative weights of the gastrocnemius muscle were found to be significantly lower in Group HFS than in Group N. The protein concentration of the gastrocnemius muscle was also significantly lower in Group HFS than in Group N. The absolute and relative weights of the bladder were also significantly lower in Group HFS than in Group N. The contractile responses of the bladder strips to electrical field stimulation and carbachol were significantly lower in Group HFS than in Group N. The HIF1α and 8OHdG in the bladder muscle were significantly higher in Group HFS than in Group N. The HFS diet reduced bladder capacity and contractility along with the loss of the gastrocnemius muscle.

Conclusion: HFS diet promotes bladder dysfunction similar to detrusor hyperreflexia with impaired contractility.

Citing Articles

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References

McPherron A, Lawler A, Lee S . Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature. 1997; 387(6628):83-90. DOI: 10.1038/387083a0. View

Rospond B, Krakowska A, Krosniak M, Muszynska B, Opoka W . The influence of high-fat and high-sucrose feeding regimes on organ weight, body weight, and serum concentration of bioelements in rats. J Trace Elem Med Biol. 2022; 73:127020. DOI: 10.1016/j.jtemb.2022.127020. View

Chen I, Cheng J, Tong Y . Metabolic Syndrome Induced Bladder Cannabinoid Receptor Changes in the Fructose-Fed Rats. Low Urin Tract Symptoms. 2017; 10(2):198-203. DOI: 10.1111/luts.12156. View

Nomiya M, Burmeister D, Sawada N, Campeau L, Zarifpour M, Keys T . Prophylactic effect of tadalafil on bladder function in a rat model of chronic bladder ischemia. J Urol. 2012; 189(2):754-61. DOI: 10.1016/j.juro.2012.07.141. View

K S, Senthilkumar G, Sankar P, Bobby Z . Attenuation of oxidative stress, inflammation and insulin resistance by allium sativum in fructose-fed male rats. J Clin Diagn Res. 2013; 7(9):1860-2. PMC: 3809621. DOI: 10.7860/JCDR/2013/6924.3334. View

Gatineau E, Savary-Auzeloux I, Migne C, Polakof S, Dardevet D, Mosoni L . Chronic Intake of Sucrose Accelerates Sarcopenia in Older Male Rats through Alterations in Insulin Sensitivity and Muscle Protein Synthesis. J Nutr. 2015; 145(5):923-30. DOI: 10.3945/jn.114.205583. View

Nomiya M, Yamaguchi O, Akaihata H, Hata J, Sawada N, Kojima Y . Progressive vascular damage may lead to bladder underactivity in rats. J Urol. 2013; 191(5):1462-9. DOI: 10.1016/j.juro.2013.10.097. View

Merrigan J, White J, Hu Y, Stone J, Oliver J, Jones M . Differences in elbow extensor muscle characteristics between resistance-trained men and women. Eur J Appl Physiol. 2018; 118(11):2359-2366. DOI: 10.1007/s00421-018-3962-4. View

Wada N, Karnup S, Kadekawa K, Shimizu N, Kwon J, Shimizu T . Current Knowledge and Novel Frontiers in Lower Urinary Tract Dysfunction after Spinal Cord Injury: Basic Research Perspectives. Urol Sci. 2022; 33(3):101-113. PMC: 9518811. DOI: 10.4103/uros.uros_31_22. View

10.

Gaspar R, Hajagos-Toth J, Schaffer A, Kothencz A, Siska-Szabo L, Ducza E . High Fat High Sucrose Diet Modifies Uterine Contractility and Cervical Resistance in Pregnant Rats: The Roles of Sex Hormones, Adipokines and Cytokines. Life (Basel). 2022; 12(6). PMC: 9224847. DOI: 10.3390/life12060794. View

11.

Lee W, Chien C, Yu H, Lee S . Bladder dysfunction in rats with metabolic syndrome induced by long-term fructose feeding. J Urol. 2008; 179(6):2470-6. DOI: 10.1016/j.juro.2008.01.086. View

12.

Son H, Lee S, Park W, Park K, Park S, Kang M . New unstable bladder model in hypercholesterolemia rats. Urology. 2007; 69(1):186-90. DOI: 10.1016/j.urology.2006.09.062. View

13.

Dhillon R, Hasni S . Pathogenesis and Management of Sarcopenia. Clin Geriatr Med. 2016; 33(1):17-26. PMC: 5127276. DOI: 10.1016/j.cger.2016.08.002. View

14.

Severinsen M, Pedersen B . Muscle-Organ Crosstalk: The Emerging Roles of Myokines. Endocr Rev. 2020; 41(4). PMC: 7288608. DOI: 10.1210/endrev/bnaa016. View

15.

Lee W, Leu S, Wu K, Tain Y, Chuang Y, Chan J . Tadalafil ameliorates bladder overactivity by restoring insulin-activated detrusor relaxation via the bladder mucosal IRS/PI3K/AKT/eNOS pathway in fructose-fed rats. Sci Rep. 2021; 11(1):8202. PMC: 8050041. DOI: 10.1038/s41598-021-87505-3. View

16.

Pedersen B, Febbraio M . Muscles, exercise and obesity: skeletal muscle as a secretory organ. Nat Rev Endocrinol. 2012; 8(8):457-65. DOI: 10.1038/nrendo.2012.49. View

17.

Fourny N, Lan C, Bernard M, Desrois M . Male and Female Rats Have Different Physiological Response to High-Fat High-Sucrose Diet but Similar Myocardial Sensitivity to Ischemia-Reperfusion Injury. Nutrients. 2021; 13(9). PMC: 8472056. DOI: 10.3390/nu13092914. View

18.

Majima T, Funahashi Y, Matsukawa Y, Inoue S, Sassa N, Kato M . Investigation of the relationship between bladder function and sarcopenia using pressure flow studies in elderly male patients. Neurourol Urodyn. 2019; 38(5):1417-1422. DOI: 10.1002/nau.24001. View

19.

Pedersen B . Physical activity and muscle-brain crosstalk. Nat Rev Endocrinol. 2019; 15(7):383-392. DOI: 10.1038/s41574-019-0174-x. View

20.

Kadekawa K, Yoshimura N, Majima T, Wada N, Shimizu T, Birder L . Characterization of bladder and external urethral activity in mice with or without spinal cord injury--a comparison study with rats. Am J Physiol Regul Integr Comp Physiol. 2016; 310(8):R752-8. PMC: 4867409. DOI: 10.1152/ajpregu.00450.2015. View