High-resolution Cryo-EM Structure of the Pseudomonas Bacteriophage E217

Overview

Journal Nat Commun

Specialty Biology

Date 2023 Jul 8

PMID 37422479

Authors

Fenglin Li

Chun-Feng David Hou

Ravi K Lokareddy

Ruoyu Yang

Francesca Forti

Federica Briani

Gino Cingolani

Affiliations

Soon will be listed here.

Abstract

E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 Å and 4.5 Å resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4.

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