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The Purification and Complete Amino Acid Sequence of the 9000-Mr Ca2+-binding Protein from Rat Placenta. Identity with the Vitamin D-dependent Intestinal Ca2+-binding Protein

Overview
Journal Biochem J
Specialty Biochemistry
Date 1986 Apr 15
PMID 3741407
Citations 10
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Abstract

A 9000-Mr Ca2+-binding protein was isolated from rat placenta and purified to homogeneity by h.p.l.c. procedures. The complete amino acid sequence was established for the 78-residue placental protein. A sequence analysis of a minor component of the rat intestinal Ca2+-binding protein (residues 4-78) and a tryptic peptide (residues 55-74), both purified by h.p.l.c., showed both proteins to be identical. Thus this placental 9000-Mr Ca2+-binding protein is the same gene product as the intestinal Ca2+-binding protein whose synthesis is dependent on vitamin D.

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