Study on Diversity, Nitrogen-fixing Capacity, and Heavy Metal Tolerance of Culturable Rhizobia in the Vanadium-titanium Magnetite Tailings

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2023 Jul 5

PMID 37405163

Authors

Tian Shen

Ruimin Jin

Jing Yan

Xiran Cheng

Lan Zeng

Qiang Chen

Yunfu Gu

Likou Zou

Ke Zhao

Quanju Xiang

Petri Penttinen

Menggen Ma

Shuangcheng Li

Ting Zou

Xiumei Yu

Affiliations

Soon will be listed here.

Abstract

Introduction: The diversity, nitrogen-fixing capacity and heavy metal tolerance of culturable rhizobia in symbiotic relationship with surviving in vanadium (V) - titanium (Ti) magnetite (VTM) tailings is still unknown, and the rhizobia isolates from the extreme barren VTM tailings contaminated with a variety of metals would provide available rhizobia resources for bioremediation.

Methods: plants were cultivated in pots containing the VTM tailings until root nodules formed, and then culturable rhizobia were isolated from root nodules. The diversity, nitrogen-fixing capacity and heavy metal tolerance of rhizobia were performed.

Results: Among 57 rhizobia isolated from these nodules, only twenty strains showed different levels of tolerance to copper (Cu), nickel (Ni), manganese (Mn) and zinc (Zn), especially strains PP1 and PP76 showing high tolerance against these four heavy metals. Based on the phylogenetic analysis of 16S rRNA and four house-keeping genes (, , , ), twelve isolates were identified as , four as , three as and one as . Some rhizobia isolates showed a high nitrogen-fixing capacity and promoted growth by increasing nitrogen content by 10%-145% in aboveground plant part and 13%-79% in the root. PP1 showed the strongest capacity of nitrogen fixation, plant growth promotion and resistance to heavy metals, which provided effective rhizobia strains for bioremediation of VTM tailings or other contaminated soils. This study demonstrated that there are at least three genera of culturable rhizobia in symbiosis with in VTM tailings.

Discussion: Abundant culturable rhizobia with the capacity of nitrogen fixation, plant growth promotion and resistance to heavy metals survived in VTM tailings, indicating more valuable functional microbes could be isolated from extreme soil environments such as VTM tailings.

Citing Articles

Evaluation of the control efficacy of antagonistic bacteria from V-Ti magnetite mine tailings on kiwifruit brown spots in pot and field experiments.

Cui Y, Zhu Y, Dong G, Li Y, Xu J, Cheng Z Front Microbiol. 2024; 15:1280333.

PMID: 38533328 PMC: 10963537. DOI: 10.3389/fmicb.2024.1280333.

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