Effects of Transgenic on Root Development, Leaf Morphology and Stress Resistance

Overview

Journal Breed Sci

Date 2023 Jul 5

PMID 37404353

Authors

Lijiao Fan

Dongshan Wei

Xingwang Yu

Fengqiang Yu

Jiameng Wang

Guirong Sun

Alatengsuhe

Li Zhang

Guosheng Zhang

Haifeng Yang

Affiliations

Soon will be listed here.

Abstract

To identify the function of the gene and its response to salt and drought stress, the gene was transformed into by the Agrobacterium-mediated leaf disc method, and the phenotypic, physiological changes and related genes expression of transgenic lines were analyzed. The results showed that the number and length of roots of transgenic lines increased significantly. The leaves of transgenic lines curled inward. Under salt and simulated drought stress, the transgenic lines showed improved tolerance to salt and drought. The activities of SOD, POD, CAT and proline content in the transgenic lines were significantly increased, and the reduction rates of total chlorophyll and MDA content were significantly decreased, which indicated that the transgenic lines showed strong physiological responses under stress. Meanwhile, the gene expression of , , and were significantly upregulated, and the gene expression of was significantly downregulated, which preliminarily verified the stress regulation mechanism that might activate. The above results showed that the gene could promote root development, make leaf morphology curl, and enhance tolerance to stress.

References

Chen K, Li F, Xu C, Zhang S, Fu C . [An efficient macro-method of genomic DNA isolation from Actinidia chinensis leaves]. Yi Chuan. 2005; 26(4):529-31. View

Nanjo T, Kobayashi M, Yoshiba Y, Kakubari Y, Yamaguchi-Shinozaki K, Shinozaki K . Antisense suppression of proline degradation improves tolerance to freezing and salinity in Arabidopsis thaliana. FEBS Lett. 1999; 461(3):205-10. DOI: 10.1016/s0014-5793(99)01451-9. View

Chen X, Cheng J, Chen L, Zhang G, Huang H, Zhang Y . Auxin-Independent NAC Pathway Acts in Response to Explant-Specific Wounding and Promotes Root Tip Emergence during de Novo Root Organogenesis in Arabidopsis. Plant Physiol. 2016; 170(4):2136-45. PMC: 4825153. DOI: 10.1104/pp.15.01733. View

Berthomieu P, Conejero G, Nublat A, Brackenbury W, Lambert C, Savio C . Functional analysis of AtHKT1 in Arabidopsis shows that Na(+) recirculation by the phloem is crucial for salt tolerance. EMBO J. 2003; 22(9):2004-14. PMC: 156079. DOI: 10.1093/emboj/cdg207. View

Wang Y, Duan L, Lu M, Li Z, Wang M, Zhai Z . Expression of NAC1 up-stream regulatory region and its relationship to the lateral root initiation induced by gibberellins and auxins. Sci China C Life Sci. 2006; 49(5):429-35. DOI: 10.1007/s11427-006-2021-2. View

Funck D, Eckard S, Muller G . Non-redundant functions of two proline dehydrogenase isoforms in Arabidopsis. BMC Plant Biol. 2010; 10:70. PMC: 3095344. DOI: 10.1186/1471-2229-10-70. View

Huh S, Lee S, Kim H, Paek K . ATAF2, a NAC transcription factor, binds to the promoter and regulates NIT2 gene expression involved in auxin biosynthesis. Mol Cells. 2012; 34(3):305-13. PMC: 3887843. DOI: 10.1007/s10059-012-0122-2. View

Liang X, Shalapy M, Zhao S, Liu J, Wang J . A stress-responsive transcription factor PeNAC1 regulating beta-D-glucan biosynthetic genes enhances salt tolerance in oat. Planta. 2021; 254(6):130. DOI: 10.1007/s00425-021-03770-6. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

10.

Guo H, Xie Q, Fei J, Chua N . MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for arabidopsis lateral root development. Plant Cell. 2005; 17(5):1376-86. PMC: 1091761. DOI: 10.1105/tpc.105.030841. View

11.

Hu R, Qi G, Kong Y, Kong D, Gao Q, Zhou G . Comprehensive analysis of NAC domain transcription factor gene family in Populus trichocarpa. BMC Plant Biol. 2010; 10:145. PMC: 3017804. DOI: 10.1186/1471-2229-10-145. View

12.

. A simple and general method for transferring genes into plants. Science. 1985; 227(4691):1229-31. DOI: 10.1126/science.227.4691.1229. View

13.

Liu F, Sun Z, Cui D, Du B, Wang C, Chen S . [Cloning of E. coli mtl-D gene and its expression in transgenic Balizhuangyang (Populus)]. Yi Chuan Xue Bao. 2000; 27(5):428-33. View

14.

Le D, Nishiyama R, Watanabe Y, Mochida K, Yamaguchi-Shinozaki K, Shinozaki K . Genome-wide survey and expression analysis of the plant-specific NAC transcription factor family in soybean during development and dehydration stress. DNA Res. 2011; 18(4):263-76. PMC: 3158466. DOI: 10.1093/dnares/dsr015. View

15.

Sun L, Li D, Zhang H, Song F . [Functions of NAC transcription factors in biotic and abiotic stress responses in plants]. Yi Chuan. 2012; 34(8):993-1002. DOI: 10.3724/sp.j.1005.2012.00993. View

16.

Li X, Zheng Y, Xing Q, Ardiansyah R, Zhou H, Ali S . Ectopic expression of the transcription factor CUC2 restricts growth by cell cycle inhibition in leaves. Plant Signal Behav. 2020; 15(1):1706024. PMC: 7012148. DOI: 10.1080/15592324.2019.1706024. View

17.

Yoshiba Y, Kiyosue T, Katagiri T, Ueda H, Mizoguchi T, Yamaguchi-Shinozaki K . Correlation between the induction of a gene for delta 1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress. Plant J. 1995; 7(5):751-60. DOI: 10.1046/j.1365-313x.1995.07050751.x. View

18.

Li J, Guo G, Guo W, Guo G, Tong D, Ni Z . miRNA164-directed cleavage of ZmNAC1 confers lateral root development in maize (Zea mays L.). BMC Plant Biol. 2012; 12:220. PMC: 3554535. DOI: 10.1186/1471-2229-12-220. View

19.

Nuruzzaman M, Manimekalai R, Sharoni A, Satoh K, Kondoh H, Ooka H . Genome-wide analysis of NAC transcription factor family in rice. Gene. 2010; 465(1-2):30-44. DOI: 10.1016/j.gene.2010.06.008. View

20.

Qiu Q, Guo Y, Dietrich M, Schumaker K, Zhu J . Regulation of SOS1, a plasma membrane Na+/H+ exchanger in Arabidopsis thaliana, by SOS2 and SOS3. Proc Natl Acad Sci U S A. 2002; 99(12):8436-41. PMC: 123085. DOI: 10.1073/pnas.122224699. View