Converting a Probe-based Fluorescence System into an Easy-to-use Adjunct for the Detection of Parathyroid Glands Accidentally Resected Intraoperatively

Overview

Journal Langenbecks Arch Surg

Specialty General Surgery

Date 2023 Jul 1

PMID 37393198

Authors

Wei Chen

Ru Zhang

Ruijie Yang

Jie Hu

John E Phay

Peng Liu

Xiaopeng Ma

Ronald X Xu

Affiliations

Soon will be listed here.

Abstract

Purpose: The reported threshold of a near-infrared fluorescence detection probe (FDP) for judging parathyroid glands (PGs) is based on the autofluorescence intensity relative to other non-PG tissues, making it unreliable when not enough reference tissues are measured. We aim to convert FDP into a more convenient tool for identifying accidentally resected PGs by quantitative measurements of autofluorescence in resected tissues.

Methods: It was a prospective study approved by the Institutional Review Board. The research was divided into two stages: (1) In order to calibrate the novel FDP system, autofluorescence intensity of different in / ex vivo tissues was measured and the optimal threshold was obtained using receiver operating characteristic (ROC) curve. (2) To further validate the effectiveness of the new system, detection rates of incidental resected PGs by pathology in the control group and by FDP in the experimental group were compared.

Results: Autofluorescence of PGs was significantly higher than that of non-PG tissue (43 patients, Mann-Whitney U test, p < 0.0001). An optimal threshold of sensitivity / specificity (78.8% and 85.1%) for discriminating PGs was obtained. The detection rates of experimental group (20 patients) and control group (33 patients) are 5.0% and 6.1% respectively (one-tailed Fisher's exact test, p = 0.6837), indicating the novel FDP system can achieve a similar proportion of PG detection compared with pathological examinations.

Conclusions: The novel FDP system can be used as an easy-to-use adjunct for detecting PG accidentally resected intraoperatively before the tissues are sent for frozen sections during thyroidectomy surgeries.

Trial Registration: Registration number: ChiCTR2200057957.

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