Identification of BST2 As a Conjunctival Epithelial Stem/progenitor Cell Marker

Overview

Journal iScience

Publisher Cell Press

Date 2023 Jun 30

PMID 37389178

Authors

Masahiro Kitao

Ryuhei Hayashi

Kimihito Nomi

Reiko Kobayashi

Tomohiko Katayama

Hiroshi Takayanagi

Akiko Oguchi

Yasuhiro Murakawa

Kohji Nishida

Affiliations

Soon will be listed here.

Abstract

The conjunctival epithelium consists of conjunctival epithelial cells and goblet cells derived from conjunctival epithelial stem/progenitor cells. However, the source of these cells is not well known because no specific markers for conjunctival epithelial stem/progenitor cells have been discovered. Therefore, to identify conjunctival epithelial stem/progenitor cell markers, we performed single-cell RNA sequencing of a conjunctival epithelial cell population derived from human-induced pluripotent stem cells (hiPSCs). The following conjunctival epithelial markers were identified: BST2, SLC2A3, AGR2, TMEM54, OLR1, and TRIM29. Notably, BST2 was strongly positive in the basal conjunctival epithelium, which is thought to be rich in stem/progenitor cells. Moreover, BST2 was able to sort conjunctival epithelial stem/progenitor cells from hiPSC-derived ocular surface epithelial cell populations. BST2-positive cells were highly proliferative and capable of successfully generating conjunctival epithelial sheets containing goblet cells. In conclusion, BST2 has been identified as a specific marker of conjunctival epithelial stem/progenitor cells.

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