Effect of the Peptide Calcium Channel Blocker ω-hexatoxin-Hv1a on Cell Death During Ischemia/Reperfusion

Overview

Journal Sovrem Tekhnologii Med

Specialties Biomedical Engineering
General Medicine

Date 2023 Jun 30

PMID 37388753

Authors

E V Iurova

E A Beloborodov

Yu V Saenko

D E Sugak

A N Fomin

S M Slesarev

Ye S Pogodina

Affiliations

Soon will be listed here.

Abstract

Materials And Methods: In this study, we used CHO-K1 epithelial cell culture. Changes in apoptosis, necrosis, cell index, and calcium ion concentration were assessed when modeling ischemia/reperfusion processes with the addition of a calcium channel blocker toxin. Ischemic and reperfusion injury was achieved by oxygen and nutrient deprivation followed by reperfusion in a complete nutrient medium. The measurements were performed using a multimodal plate reader-fluorimeter.

Results: An increase in apoptosis, necrosis, and the concentration of calcium ions was recorded when modeling ischemia/reperfusion processes. A decrease in the level of apoptosis and necrosis, as well as the concentration of calcium ions to a physiological level or a level close to physiological, was noted when the toxin was added at a concentration of 50 nM at the reperfusion stage. The cell index showed a faster restoration in the presence of the toxin.

Conclusion: The experimental data confirm the hypothesis of a beneficial effect of peptide calcium channel blockers on the state of epithelial cells during reperfusion after ischemia and can be considered for further study as a strategy for organ adaptation before reperfusion.

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