Reorientation of INO80 on Hexasomes Reveals Basis for Mechanistic Versatility

Overview

Journal Science

Specialty Science

Date 2023 Jun 29

PMID 37384669

Authors

Hao Wu

Elise N Munoz

Laura J Hsieh

Un Seng Chio

Muryam A Gourdet

Geeta J Narlikar

Yifan Cheng

Affiliations

Soon will be listed here.

Abstract

Unlike other chromatin remodelers, INO80 preferentially mobilizes hexasomes, which can form during transcription. Why INO80 prefers hexasomes over nucleosomes remains unclear. Here, we report structures of INO80 bound to a hexasome or a nucleosome. INO80 binds the two substrates in substantially different orientations. On a hexasome, INO80 places its ATPase subunit, Ino80, at superhelical location -2 (SHL -2), in contrast to SHL -6 and SHL -7, as previously seen on nucleosomes. Our results suggest that INO80 action on hexasomes resembles action by other remodelers on nucleosomes such that Ino80 is maximally active near SHL -2. The SHL -2 position also plays a critical role for nucleosome remodeling by INO80. Overall, the mechanistic adaptations used by INO80 for preferential hexasome sliding imply that subnucleosomal particles play considerable regulatory roles.

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