CH02 Peptide Promotes Expansion of Umbilical Cord Blood-derived CD34 Hematopoietic Stem/progenitor Cells

Overview

Journal Acta Biochim Biophys Sin (Shanghai)

Specialties Biochemistry
Biophysics

Date 2023 Jun 29

PMID 37381672

Authors

Yiqi Yang

Bihui Zhang

Junye Xie

Jingsheng Li

Jia Liu

Rongzhan Liu

Linhao Zhang

Jinting Zhang

Zijian Su

Fu Li

Leisheng Zhang

An Hong

Xiaojia Chen

Affiliations

Soon will be listed here.

Abstract

Umbilical cord blood (UCB) is an advantageous source for hematopoietic stem/progenitor cell (HSPC) transplantation, yet the current strategies for large-scale and cost-effective UCB-HSPC preparation are still unavailable. To overcome these obstacles, we systematically evaluate the feasibility of our newly identified CH02 peptide for expansion of CD34 UCB-HSPCs. We herein report that the CH02 peptide is specifically enriched in HSPC proliferation via activating the FLT3 signaling. Notably, the CH02-based cocktails are adequate for boosting 12-fold expansion of UCB-HSPCs. Meanwhile, CH02-preconditioned UCB-HSPCs manifest preferable efficacy upon wound healing in diabetic mice via bidirectional orchestration of proinflammatory and anti-inflammatory factors. Together, our data indicate the advantages of the CH02-based strategy for expansion of CD34 UCB-HSPCs, which will provide new strategies for further development of large-scale HSPC preparation for clinical purposes.

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