Lignin Nanoparticles with Entrapped Spp. Essential Oils for the Control of Wood-Rot Fungi

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2023 Jun 28

PMID 37376359

Authors

Florian Zikeli

Anna Maria Vettraino

Margherita Biscontri

Sara Bergamasco

Cleofe Palocci

Miha Humar

Manuela Romagnoli

Affiliations

Soon will be listed here.

Abstract

After decades of utilization of fossil-based and environmentally hazardous compounds for wood preservation against fungal attack, there is a strong need to substitute those compounds with bio-based bioactive solutions, such as essential oils. In this work, lignin nanoparticles containing four essential oils from thyme species (, , , and Demeter) were applied as biocides in in vitro experiments to test their anti-fungal effect against two white-rot fungi ( and ) and two brown-rot fungi ( and ). Entrapment of essential oils provided a delayed release over a time frame of 7 days from the lignin carrier matrix and resulted in lower minimum inhibitory concentrations of the essential oils against the brown-rot fungi (0.30-0.60 mg/mL), while for the white-rot fungi, identical concentrations were determined compared with free essential oils (0.05-0.30 mg/mL). Fourier Transform infrared (FTIR) spectroscopy was used to assess the fungal cell wall changes in the presence of essential oils in the growth medium. The results regarding brown-rot fungi present a promising approach for a more effective and sustainable utilization of essential oils against this class of wood-rot fungi. In the case of white-rot fungi, lignin nanoparticles, as essential oils delivery vehicles, still need optimization in their efficacy.

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References

Felsociova S, Vukovic N, Jezowski P, Kacaniova M . Antifungal activity of selected volatile essential oils against sp. Open Life Sci. 2021; 15(1):511-521. PMC: 7874545. DOI: 10.1515/biol-2020-0045. View

Skotti E, Kountouri S, Bouchagier P, Tsitsigiannis D, Polissiou M, Tarantilis P . FTIR spectroscopic evaluation of changes in the cellular biochemical composition of the phytopathogenic fungus Alternaria alternata induced by extracts of some Greek medicinal and aromatic plants. Spectrochim Acta A Mol Biomol Spectrosc. 2014; 127:463-72. DOI: 10.1016/j.saa.2014.02.113. View

Henn K, Forsman N, Zou T, Osterberg M . Colloidal Lignin Particles and Epoxies for Bio-Based, Durable, and Multiresistant Nanostructured Coatings. ACS Appl Mater Interfaces. 2021; 13(29):34793-34806. PMC: 8397241. DOI: 10.1021/acsami.1c06087. View

Szeghalmi A, Kaminskyj S, Gough K . A synchrotron FTIR microspectroscopy investigation of fungal hyphae grown under optimal and stressed conditions. Anal Bioanal Chem. 2006; 387(5):1779-89. DOI: 10.1007/s00216-006-0850-2. View

Ochoa-Velasco C, Navarro-Cruz A, Vera-Lopez O, Palou E, Avila-Sosa R . Growth modeling to control (in vitro) Fusarium verticillioides and Rhizopus stolonifer with thymol and carvacrol. Rev Argent Microbiol. 2017; 50(1):70-74. DOI: 10.1016/j.ram.2016.11.010. View

Winska K, Maczka W, Lyczko J, Grabarczyk M, Czubaszek A, Szumny A . Essential Oils as Antimicrobial Agents-Myth or Real Alternative?. Molecules. 2019; 24(11). PMC: 6612361. DOI: 10.3390/molecules24112130. View

Vrenna G, Artini M, Ragno R, Relucenti M, Fiscarelli E, Tuccio Guarna Assanti V . Anti-Virulence Properties of Essential Oil against Clinical Isolates from Cystic Fibrosis Patients. Microorganisms. 2021; 9(11). PMC: 8623622. DOI: 10.3390/microorganisms9112257. View

Cosentino S, Tuberoso C, Pisano B, Satta M, Mascia V, Arzedi E . In-vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Lett Appl Microbiol. 1999; 29(2):130-5. DOI: 10.1046/j.1472-765x.1999.00605.x. View

Kijpornyongpan T, Schwartz A, Yaguchi A, Salvachua D . Systems biology-guided understanding of white-rot fungi for biotechnological applications: A review. iScience. 2022; 25(7):104640. PMC: 9272384. DOI: 10.1016/j.isci.2022.104640. View

10.

Beisl S, Friedl A, Miltner A . Lignin from Micro- to Nanosize: Applications. Int J Mol Sci. 2017; 18(11). PMC: 5713336. DOI: 10.3390/ijms18112367. View

11.

Vinciguerra V, Rojas F, Tedesco V, Giusiano G, Angiolella L . Chemical characterization and antifungal activity of , essential oils and carvacrol against . Nat Prod Res. 2018; 33(22):3273-3277. DOI: 10.1080/14786419.2018.1468325. View

12.

Sipponen M, Lange H, Ago M, Crestini C . Understanding Lignin Aggregation Processes. A Case Study: Budesonide Entrapment and Stimuli Controlled Release from Lignin Nanoparticles. ACS Sustain Chem Eng. 2018; 6(7):9342-9351. PMC: 6156105. DOI: 10.1021/acssuschemeng.8b01652. View

13.

Vieira F, Magina S, Evtuguin D, Barros-Timmons A . Lignin as a Renewable Building Block for Sustainable Polyurethanes. Materials (Basel). 2022; 15(17). PMC: 9457695. DOI: 10.3390/ma15176182. View

14.

Nazzaro F, Fratianni F, De Martino L, Coppola R, De Feo V . Effect of essential oils on pathogenic bacteria. Pharmaceuticals (Basel). 2013; 6(12):1451-74. PMC: 3873673. DOI: 10.3390/ph6121451. View

15.

Bergamasco S, Tamantini S, Zikeli F, Vinciguerra V, Scarascia Mugnozza G, Romagnoli M . Synthesis and Characterizations of Eco-Friendly Organosolv Lignin-Based Polyurethane Coating Films for the Coating Industry. Polymers (Basel). 2022; 14(3). PMC: 8839005. DOI: 10.3390/polym14030416. View

16.

Dzurendova S, Zimmermann B, Kohler A, Tafintseva V, Slany O, certik M . Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi. PLoS One. 2020; 15(6):e0234870. PMC: 7307774. DOI: 10.1371/journal.pone.0234870. View

17.

Broda M . Natural Compounds for Wood Protection against Fungi-A Review. Molecules. 2020; 25(15). PMC: 7435604. DOI: 10.3390/molecules25153538. View

18.

Balakshin M, Capanema E, Sulaeva I, Schlee P, Huang Z, Feng M . New Opportunities in the Valorization of Technical Lignins. ChemSusChem. 2020; 14(4):1016-1036. DOI: 10.1002/cssc.202002553. View

19.

Sharma A, Rajendran S, Srivastava A, Sharma S, Kundu B . Antifungal activities of selected essential oils against Fusarium oxysporum f. sp. lycopersici 1322, with emphasis on Syzygium aromaticum essential oil. J Biosci Bioeng. 2016; 123(3):308-313. DOI: 10.1016/j.jbiosc.2016.09.011. View

20.

Tamantini S, Bergamasco S, Zikeli F, Humar M, Cavalera M, Romagnoli M . Cellulose Nano Crystals (CNC) as Additive for a Bio-Based Waterborne Acrylic Wood Coating: Decay, Artificial Weathering, Physical and Chemical Tests. Nanomaterials (Basel). 2023; 13(3). PMC: 9921673. DOI: 10.3390/nano13030442. View