Efficacy of JKTJ-3 in Suppression of Damping-Off of Watermelon

Overview

Journal Microorganisms

Specialty Microbiology

Date 2023 Jun 28

PMID 37374863

Authors

Mihong Ge

Xiang Cai

Dehuan Wang

Huan Liang

Juhong Zhu

Guoqing Li

Xianfeng Shi

Affiliations

Soon will be listed here.

Abstract

Damping-off caused by () is one of the most destructive diseases for watermelon seedlings. Application of biological control agents against has attracted the attention of many researchers for a long time. In this study, the actinomycetous isolate JKTJ-3 with strong and broad-spectrum antifungal activity was screened from 23 bacterial isolates. Based on the morphological, cultural, physiological, and biochemical characteristics as well as the feature of 16S rDNA sequence, isolate JKTJ-3 was identified as . We investigated the biocontrol efficacy of isolate JKTJ-3 and its metabolites. The results revealed that seed and substrate treatments with JKTJ-3 cultures showed a significant inhibitory effect on watermelon damping-off disease. Seed treatment with the JKTJ-3 cultural filtrates (CF) displayed higher control efficacy compared to the fermentation cultures (FC). Treatment of the seeding substrate with the wheat grain cultures (WGC) of JKTJ-3 exhibited better control efficacy than that of the seeding substrate with the JKTJ-3 CF. Moreover, the JKTJ-3 WGC showed the preventive effect on suppression of the disease, and the efficacy increased with increase in the inoculation interval between the WGC and . Production of the antifungal metabolite actinomycin D by isolate JKTJ-3 and cell-wall-degrading enzymes such as β-1,3-glucanase and chitosanase were probably the mechanisms for effective control of watermelon damping-off. It was shown for the first time that can produce anti-oomycete substances including chitinase and actinomycin D. This is the first report about used as biocontrol agent against watermelon damping-off caused by .

Citing Articles

Biotechnological potential of actinomycetes in the 21st century: a brief review.

Rodrigues R, Souza A, Feitoza M, Alves T, Barbosa A, Santiago S Antonie Van Leeuwenhoek. 2024; 117(1):82.

PMID: 38789815 DOI: 10.1007/s10482-024-01964-y.

Diversity and antimicrobial activity of the tropical ant-derived actinomycetes isolated from Thailand.

Tunvongvinis T, Jaitrong W, Samung Y, Tanasupawat S, Phongsopitanun W AIMS Microbiol. 2024; 10(1):68-82.

PMID: 38525037 PMC: 10955170. DOI: 10.3934/microbiol.2024005.

References

Dukare A, Paul S, Nambi V, Gupta R, Singh R, Sharma K . Exploitation of microbial antagonists for the control of postharvest diseases of fruits: a review. Crit Rev Food Sci Nutr. 2018; 59(9):1498-1513. DOI: 10.1080/10408398.2017.1417235. View

Georgakopoulos D, Fiddaman P, Leifert C, Malathrakis N . Biological control of cucumber and sugar beet damping-off caused by Pythium ultimum with bacterial and fungal antagonists. J Appl Microbiol. 2002; 92(6):1078-86. DOI: 10.1046/j.1365-2672.2002.01658.x. View

Bhatti A, Haq S, Bhat R . Actinomycetes benefaction role in soil and plant health. Microb Pathog. 2017; 111:458-467. DOI: 10.1016/j.micpath.2017.09.036. View

Zajkowicz A, Gdowicz-Klosok A, Krzesniak M, Scieglinska D, Rusin M . Actinomycin D and nutlin-3a synergistically promote phosphorylation of p53 on serine 46 in cancer cell lines of different origin. Cell Signal. 2015; 27(9):1677-87. DOI: 10.1016/j.cellsig.2015.05.005. View

Labeda D, Goodfellow M, Brown R, Ward A, Lanoot B, Vanncanneyt M . Phylogenetic study of the species within the family Streptomycetaceae. Antonie Van Leeuwenhoek. 2011; 101(1):73-104. DOI: 10.1007/s10482-011-9656-0. View

Hong T, Cheng C, Huang J, Meng M . Isolation and biochemical characterization of an endo-1,3-beta-glucanase from Streptomyces sioyaensis containing a C-terminal family 6 carbohydrate-binding module that binds to 1,3-beta-glucan. Microbiology (Reading). 2002; 148(Pt 4):1151-1159. DOI: 10.1099/00221287-148-4-1151. View

Halo B, Al-Yahyai R, Maharachchikumbura S, Al-Sadi A . Talaromyces variabilis interferes with Pythium aphanidermatum growth and suppresses Pythium-induced damping-off of cucumbers and tomatoes. Sci Rep. 2019; 9(1):11255. PMC: 6677756. DOI: 10.1038/s41598-019-47736-x. View

Felsenstein J . CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP. Evolution. 2017; 39(4):783-791. DOI: 10.1111/j.1558-5646.1985.tb00420.x. View

Tamura K, Nei M . Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993; 10(3):512-26. DOI: 10.1093/oxfordjournals.molbev.a040023. View

10.

Lewis J, Larkin R, Rogers D . A Formulation of Trichoderma and Gliocladium to Reduce Damping-off Caused by Rhizoctonia solani and Saprophytic Growth of the Pathogen in Soilless Mix. Plant Dis. 2019; 82(5):501-506. DOI: 10.1094/PDIS.1998.82.5.501. View

11.

Roberts D, Selmer K, Lupitskyy R, Rice C, Buyer J, Maul J . Seed treatment with prodigiosin controls damping-off of cucumber caused by Pythium ultimum. AMB Express. 2021; 11(1):10. PMC: 7788126. DOI: 10.1186/s13568-020-01169-2. View

12.

Komaki H . Reclassification of and as later heterotypic synonyms of . Int J Syst Evol Microbiol. 2021; 71(2). DOI: 10.1099/ijsem.0.004638. View

13.

Valois D, Fayad K, Barasubiye T, Garon M, Dery C, Brzezinski R . Glucanolytic Actinomycetes Antagonistic to Phytophthora fragariae var. rubi, the Causal Agent of Raspberry Root Rot. Appl Environ Microbiol. 1996; 62(5):1630-5. PMC: 1388851. DOI: 10.1128/aem.62.5.1630-1635.1996. View

14.

Mohd Hata E, Yusof M, Zulperi D . Induction of Systemic Resistance against Bacterial Leaf Streak Disease and Growth Promotion in Rice Plant by Streptomyces shenzhenesis TKSC3 and Streptomyces sp. SS8. Plant Pathol J. 2021; 37(2):173-181. PMC: 8053841. DOI: 10.5423/PPJ.OA.05.2020.0083. View

15.

Goodfellow M, Kumar Y, Labeda D, Sembiring L . The Streptomyces violaceusniger clade: a home for Streptomycetes with rugose ornamented spores. Antonie Van Leeuwenhoek. 2007; 92(2):173-99. DOI: 10.1007/s10482-007-9146-6. View

16.

Benhamou N, Belanger R . Induction of systemic resistance to Pythium damping-off in cucumber plants by benzothiadiazole: ultrastructure and cytochemistry of the host response. Plant J. 2004; 14(1):13-21. DOI: 10.1046/j.1365-313X.1998.00088.x. View

17.

Roberts D, Lakshman D, McKenna L, Emche S, Maul J, Bauchan G . Seed Treatment with Ethanol Extract of Serratia marcescens is Compatible with Trichoderma Isolates for Control of Damping-off of Cucumber Caused by Pythium ultimum. Plant Dis. 2019; 100(7):1278-1287. DOI: 10.1094/PDIS-09-15-1039-RE. View

18.

Jones K . FRESH ISOLATES OF ACTINOMYCETES IN WHICH THE PRESENCE OF SPOROGENOUS AERIAL MYCELIA IS A FLUCTUATING CHARACTERISTIC. J Bacteriol. 1949; 57(2):141-5. PMC: 385488. DOI: 10.1128/jb.57.2.141-145.1949. View

19.

Njoroge S, Riley M, Keinath A . Effect of Incorporation of Brassica spp. Residues on Population Densities of Soilborne Microorganisms and on Damping-off and Fusarium Wilt of Watermelon. Plant Dis. 2019; 92(2):287-294. DOI: 10.1094/PDIS-92-2-0287. View

20.

Arora H, Sharma A, Sharma S, Haron F, Gafur A, Sayyed R . Damping-Off and Root Rot of L.: Impacts, Diagnosis, and Management. Microorganisms. 2021; 9(4). PMC: 8069622. DOI: 10.3390/microorganisms9040823. View