Light-Dependent Expression and Promoter Methylation of the Genes Encoding Succinate Dehydrogenase, Fumarase, and NAD-Malate Dehydrogenase in Maize ( L.) Leaves

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Jun 28

PMID 37373359

Authors

Alexander T Eprintsev

Dmitry N Fedorin

Abir U Igamberdiev

Affiliations

Soon will be listed here.

Abstract

The expression and methylation of promoters of the genes encoding succinate dehydrogenase, fumarase, and NAD-malate dehydrogenase in maize ( L.) leaves depending on the light regime were studied. The genes encoding the catalytic subunits of succinate dehydrogenase showed suppression of expression upon irradiation by red light, which was abolished by far-red light. This was accompanied by an increase in promoter methylation of the gene encoding the flavoprotein subunit A, while methylation was low for encoding the iron-sulfur subunit B under all conditions. The expression of and encoding the anchoring subunits C and D was not affected by red light. The expression of encoding the mitochondrial form of fumarase was regulated by red and far-red light via methylation of its promoter. Only one gene encoding the mitochondrial NAD-malate dehydrogenase gene () was regulated by red and far-red light, while the second gene () did not respond to irradiation, and neither gene was controlled by promoter methylation. It is concluded that the dicarboxylic branch of the tricarboxylic acid cycle is regulated by light via the phytochrome mechanism, and promoter methylation is involved with the flavoprotein subunit of succinate dehydrogenase and the mitochondrial fumarase.

Citing Articles

The Role of Glutamate Metabolism and the GABA Shunt in Bypassing the Tricarboxylic Acid Cycle in the Light.

Eprintsev A, Anokhina G, Shakhov Z, Moskvina P, Igamberdiev A Int J Mol Sci. 2024; 25(23).

PMID: 39684421 PMC: 11641617. DOI: 10.3390/ijms252312711.

Comparative Phenotypic and Transcriptomic Analyses Provide Novel Insights into the Molecular Mechanism of Seed Germination in Response to Low Temperature Stress in Alfalfa.

Zhang Z, Lv Y, Sun Q, Yao X, Yan H Int J Mol Sci. 2024; 25(13).

PMID: 39000350 PMC: 11241472. DOI: 10.3390/ijms25137244.

Participation of miR165a in the Phytochrome Signal Transduction in Maize ( L.) Leaves under Changing Light Conditions.

Fedorin D, Eprintsev A, Chuykova V, Igamberdiev A Int J Mol Sci. 2024; 25(11).

PMID: 38891921 PMC: 11171563. DOI: 10.3390/ijms25115733.

References

Igamberdiev A . Citrate valve integrates mitochondria into photosynthetic metabolism. Mitochondrion. 2020; 52:218-230. DOI: 10.1016/j.mito.2020.04.003. View

Igamberdiev A, Eprintsev A, Fedorin D, Popov V . Phytochrome-mediated regulation of plant respiration and photorespiration. Plant Cell Environ. 2013; 37(2):290-9. DOI: 10.1111/pce.12155. View

Al-Sady B, Ni W, Kircher S, Schafer E, Quail P . Photoactivated phytochrome induces rapid PIF3 phosphorylation prior to proteasome-mediated degradation. Mol Cell. 2006; 23(3):439-46. DOI: 10.1016/j.molcel.2006.06.011. View

Jaligot E, Beule T, Baurens F, Billotte N, Rival A . Search for methylation-sensitive amplification polymorphisms associated with the mantled variant phenotype in oil palm (Elaeis guineensis Jacq). Genome. 2004; 47(1):224-8. DOI: 10.1139/g03-085. View

Eprintsev A, Fedorin D, Karabutova L, Igamberdiev A . Expression of genes encoding subunits A and B of succinate dehydrogenase in germinating maize seeds is regulated by methylation of their promoters. J Plant Physiol. 2016; 205:33-40. DOI: 10.1016/j.jplph.2016.08.008. View

Hu J, Desai M . Light control of peroxisome proliferation during Arabidopsis photomorphogenesis. Plant Signal Behav. 2009; 3(10):801-3. PMC: 2634377. DOI: 10.4161/psb.3.10.5876. View

Castillon A, Shen H, Huq E . Blue light induces degradation of the negative regulator phytochrome interacting factor 1 to promote photomorphogenic development of Arabidopsis seedlings. Genetics. 2009; 182(1):161-71. PMC: 2674814. DOI: 10.1534/genetics.108.099887. View

Jiao Y, Lau O, Deng X . Light-regulated transcriptional networks in higher plants. Nat Rev Genet. 2007; 8(3):217-30. DOI: 10.1038/nrg2049. View

Ghosh A, Igamberdiev A, Debnath S . Tissue culture-induced DNA methylation in crop plants: a review. Mol Biol Rep. 2021; 48(1):823-841. DOI: 10.1007/s11033-020-06062-6. View

10.

Medvedeva Y, Khamis A, Kulakovskiy I, Ba-Alawi W, Bhuyan M, Kawaji H . Effects of cytosine methylation on transcription factor binding sites. BMC Genomics. 2014; 15:119. PMC: 3986887. DOI: 10.1186/1471-2164-15-119. View

11.

Pracharoenwattana I, Zhou W, Keech O, Francisco P, Udomchalothorn T, Tschoep H . Arabidopsis has a cytosolic fumarase required for the massive allocation of photosynthate into fumaric acid and for rapid plant growth on high nitrogen. Plant J. 2010; 62(5):785-95. DOI: 10.1111/j.1365-313X.2010.04189.x. View

12.

Escrich A, Cusido R, Bonfill M, Palazon J, Sanchez-Munoz R, Moyano E . The Epigenetic Regulation in Plant Specialized Metabolism: DNA Methylation Limits Paclitaxel Biotechnological Production. Front Plant Sci. 2022; 13:899444. PMC: 9305382. DOI: 10.3389/fpls.2022.899444. View

13.

Eprintsev A, Fedorin D, Cherkasskikh M, Igamberdiev A . Expression of succinate dehydrogenase and fumarase genes in maize leaves is mediated by cryptochrome. J Plant Physiol. 2017; 221:81-84. DOI: 10.1016/j.jplph.2017.12.004. View

14.

Igamberdiev A, Kleczkowski L . Thermodynamic buffering, stable non-equilibrium and establishment of the computable structure of plant metabolism. Prog Biophys Mol Biol. 2018; 146:23-36. DOI: 10.1016/j.pbiomolbio.2018.11.005. View

15.

Igamberdiev A, Bykova N . Role of organic acids in the integration of cellular redox metabolism and mediation of redox signalling in photosynthetic tissues of higher plants. Free Radic Biol Med. 2018; 122:74-85. DOI: 10.1016/j.freeradbiomed.2018.01.016. View

16.

Tomaz T, Bagard M, Pracharoenwattana I, Linden P, Lee C, Carroll A . Mitochondrial malate dehydrogenase lowers leaf respiration and alters photorespiration and plant growth in Arabidopsis. Plant Physiol. 2010; 154(3):1143-57. PMC: 2971595. DOI: 10.1104/pp.110.161612. View

17.

Abbas N, Maurya J, Senapati D, Gangappa S, Chattopadhyay S . Arabidopsis CAM7 and HY5 physically interact and directly bind to the HY5 promoter to regulate its expression and thereby promote photomorphogenesis. Plant Cell. 2014; 26(3):1036-52. PMC: 4001367. DOI: 10.1105/tpc.113.122515. View

18.

Eprintsev A, Fedorin D, Cherkasskikh M, Igamberdiev A . Regulation of expression of the mitochondrial and cytosolic forms of aconitase in maize leaves via phytochrome. Plant Physiol Biochem. 2019; 146:157-162. DOI: 10.1016/j.plaphy.2019.11.018. View

19.

Stucki J . The thermodynamic-buffer enzymes. Eur J Biochem. 1980; 109(1):257-67. DOI: 10.1111/j.1432-1033.1980.tb04791.x. View

20.

Christensen A, Quail P . Structure and expression of a maize phytochrome-encoding gene. Gene. 1989; 85(2):381-90. DOI: 10.1016/0378-1119(89)90431-9. View