High-Resolution Melting (HRM) Analysis for Rapid Molecular Identification of Species in the Greek Fish Market

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2023 Jun 28

PMID 37372435

Authors

Evanthia Chatzoglou

Nefeli Tsaousi

Apostolos P Apostolidis

Athanasios Exadactylos

Raphael Sandaltzopoulos

Ioannis A Giantsis

Georgios A Gkafas

Emmanouil E Malandrakis

Joanne Sarantopoulou

Maria Tokamani

George Triantaphyllidis

Helen Miliou

Affiliations

Soon will be listed here.

Abstract

The red porgy () and the common dentex () are Sparidae species of high commercial value, traded in the Greek market. In some cases, fish species identification from Greek fisheries is difficult for the consumer due to the strong morphological similarities with their imported counterparts or closely related species such as , , and , especially when specimens are frozen, filleted or cooked. Techniques based on DNA sequencing, such as barcoding, accurately identify species substitution incidents; however, they are time consuming and expensive. In this study, regions of mtDNA were analyzed with RFLPs, multiplex PCR and HRM in order to develop a rapid method for species identification within the Sparidae family. HRM analysis of a 113 bp region of and/or a 156 bp region of could discriminate raw or cooked samples of and from the aforementioned closely related species and specimens sampled in the Mediterranean Sea when compared to those fished in the eastern Atlantic. HRM analysis exhibited high accuracy and repeatability, revealing incidents of mislabeling. Multiple samples can be analyzed within three hours, rendering this method a useful tool in fish fraud monitoring.

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