Molecular Characterization and Transcriptional Response to TiO-GO Nanomaterial Exposure of Two Molt-related Genes in the Juvenile Prawn, Macrobrachium Rosenbergii

Overview

Journal Sci Rep

Specialty Science

Date 2023 Jun 27

PMID 37369682

Authors

Ziqi Guo

Likun Xu

Wei Wang

Wei Chen

Chunyan Ma

Fengying Zhang

Lingbo Ma

Zhiqiang Liu

Keyi Ma

Affiliations

Soon will be listed here.

Abstract

In recent years, with the widespread use of TiO-GO nanocomposite in industry, especially in the remediation of water environments, its toxic effects on aquatic organisms have received increasing attention. As molting is extremely important for crustaceans in their growth, in this study, we cloned the full-length cDNA sequences of two key genes related to molting, nuclear hormone receptor E75 (E75) and nuclear hormone receptor HR3 (HR3), in Macrobrachium rosenbergii, examined the gene expression profile, and investigated their toxicological effects on crustacean molting through nanomaterial exposure. The amino acid sequences for E75 and HR3 were respectively determined to encode 1138 and 363 acid residues. Sequence analysis showed that both E75 and HR3 contain a HOLI domain, with the E75 of M. rosenbergii being more closely related to the E75 of Palaemon carinicauda. These two genes were expressed at the highest levels in muscle, followed by hepatopancreas. The results showed that the expressions of E75 and HR3 in hepatopancreas and muscle tissues were significantly decreased after exposure to 0.1 mg/L of TiO-GO composite nanoparticles (P < 0.05). This study will serve as a foundation for subsequent research into the evaluation of nanomaterial toxicity on crustacean species.

References

Park Y, Kang S, Choi W . Exfoliated and reorganized graphite oxide on titania nanoparticles as an auxiliary co-catalyst for photocatalytic solar conversion. Phys Chem Chem Phys. 2011; 13(20):9425-31. DOI: 10.1039/c1cp20697d. View

Wang G, Feng W, Zeng X, Wang Z, Feng C, McCarthy D . Highly recoverable TiO2-GO nanocomposites for stormwater disinfection. Water Res. 2016; 94:363-370. DOI: 10.1016/j.watres.2016.02.067. View

Laudet V . Evolution of the nuclear receptor superfamily: early diversification from an ancestral orphan receptor. J Mol Endocrinol. 1998; 19(3):207-26. DOI: 10.1677/jme.0.0190207. View

Tiu S, Hult E, Yagi K, Tobe S . Farnesoic acid and methyl farnesoate production during lobster reproduction: possible functional correlation with retinoid X receptor expression. Gen Comp Endocrinol. 2011; 175(2):259-69. DOI: 10.1016/j.ygcen.2011.11.016. View

Menard A, Drobne D, Jemec A . Ecotoxicity of nanosized TiO2. Review of in vivo data. Environ Pollut. 2010; 159(3):677-84. DOI: 10.1016/j.envpol.2010.11.027. View

Guo Z, Zuo J, Liu X, Gong J, Ma K, Feng J . Effects of titanium dioxide (TiO)/activated carbon (AC) nanoparticle on the growth and immunity of the giant freshwater prawn, Macrobrachium rosenbergii: potential toxicological risks to the aquatic crustaceans. Environ Sci Pollut Res Int. 2022; 30(12):33322-33333. DOI: 10.1007/s11356-022-24555-8. View

Xie X, Zhou Y, Liu M, Tao T, Jiang Q, Zhu D . The nuclear receptor E75 from the swimming crab, Portunus trituberculatus: cDNA cloning, transcriptional analysis, and putative roles on expression of ecdysteroid-related genes. Comp Biochem Physiol B Biochem Mol Biol. 2016; 200:69-77. DOI: 10.1016/j.cbpb.2016.06.004. View

Baun A, Hartmann N, Grieger K, Kusk K . Ecotoxicity of engineered nanoparticles to aquatic invertebrates: a brief review and recommendations for future toxicity testing. Ecotoxicology. 2008; 17(5):387-95. DOI: 10.1007/s10646-008-0208-y. View

Mane-Padros D, Cruz J, Vilaplana L, Pascual N, Belles X, Martin D . The nuclear hormone receptor BgE75 links molting and developmental progression in the direct-developing insect Blattella germanica. Dev Biol. 2008; 315(1):147-60. DOI: 10.1016/j.ydbio.2007.12.015. View

10.

Ward J, Kach D . Marine aggregates facilitate ingestion of nanoparticles by suspension-feeding bivalves. Mar Environ Res. 2009; 68(3):137-42. DOI: 10.1016/j.marenvres.2009.05.002. View

11.

Techa S, Chung J . Ecdysone and retinoid-X receptors of the blue crab, Callinectes sapidus: cloning and their expression patterns in eyestalks and Y-organs during the molt cycle. Gene. 2013; 527(1):139-53. DOI: 10.1016/j.gene.2013.05.035. View

12.

Riddiford L, Hiruma K, Zhou X, Nelson C . Insights into the molecular basis of the hormonal control of molting and metamorphosis from Manduca sexta and Drosophila melanogaster. Insect Biochem Mol Biol. 2003; 33(12):1327-38. DOI: 10.1016/j.ibmb.2003.06.001. View

13.

LAM G, Jiang C, Thummel C . Coordination of larval and prepupal gene expression by the DHR3 orphan receptor during Drosophila metamorphosis. Development. 1997; 124(9):1757-69. DOI: 10.1242/dev.124.9.1757. View

14.

Li Y, Du X, Liu Z, Zhang M, Huang Y, Tian J . Two genes related to reproductive development in the juvenile prawn, Macrobrachium nipponense: Molecular characterization and transcriptional response to nanoplastic exposure. Chemosphere. 2021; 281:130827. DOI: 10.1016/j.chemosphere.2021.130827. View

15.

Guo W, Liu X, Fu K, Shi J, Lu F, Li G . Functions of nuclear receptor HR3 during larval-pupal molting in Leptinotarsa decemlineata (Say) revealed by in vivo RNA interference. Insect Biochem Mol Biol. 2015; 63:23-33. DOI: 10.1016/j.ibmb.2015.05.010. View

16.

Hannas B, LeBlanc G . Expression and ecdysteroid responsiveness of the nuclear receptors HR3 and E75 in the crustacean Daphnia magna. Mol Cell Endocrinol. 2009; 315(1-2):208-18. PMC: 3711079. DOI: 10.1016/j.mce.2009.07.013. View

17.

Jose Priya T, Li F, Zhang J, Yang C, Xiang J . Molecular characterization of an ecdysone inducible gene E75 of Chinese shrimp Fenneropenaeus chinensis and elucidation of its role in molting by RNA interference. Comp Biochem Physiol B Biochem Mol Biol. 2010; 156(3):149-57. DOI: 10.1016/j.cbpb.2010.02.004. View

18.

Guo Z, Zuo J, Feng J, Li J, Zhang S, Ma K . Impact of Titanium Dioxide-Graphene Oxide (TiO-GO) Composite Nanoparticle on the Juveniles of the Giant River Prawn, Macrobrachium rosenbergii: Physio-Biochemistry and Transcriptional Response. Mar Biotechnol (NY). 2022; 25(1):45-56. DOI: 10.1007/s10126-022-10180-6. View

19.

Zhao X, Qin Z, Liu W, Liu X, Moussian B, Ma E . Nuclear receptor HR3 controls locust molt by regulating chitin synthesis and degradation genes of Locusta migratoria. Insect Biochem Mol Biol. 2017; 92:1-11. DOI: 10.1016/j.ibmb.2017.11.001. View

20.

Carney G, Wade A, Sapra R, Goldstein E, Bender M . DHR3, an ecdysone-inducible early-late gene encoding a Drosophila nuclear receptor, is required for embryogenesis. Proc Natl Acad Sci U S A. 1997; 94(22):12024-9. PMC: 23691. DOI: 10.1073/pnas.94.22.12024. View