Culture of Cerebrospinal Fluid-contacting Neurons from Neonatal Mouse Spinal Cord

Overview

Journal Cell Tissue Bank

Specialties Anatomy & Histology
Cell Biology
General Surgery

Date 2023 Jun 27

PMID 37368142

Authors

Liang Cao

Hui-Qian Zhang

Yu-Qi He

Ping-Jiang An

Lei-Luo Yang

Wei Tan

Gang Liu

Chun-Qing Wang

Xiao-Wei Dou

Qing Li

Affiliations

Soon will be listed here.

Abstract

Cerebrospinal fluid-contacting neurons (CSF-cNs) act crucial role in chemosensory and mechanosensory function in spinal cord. Recently, CSF-cNs were found to be an immature neuron and may be involved in spinal cord injury recovery. But how to culture it and explore its function in vitro are not reported in previous research. Here, we first reported culture and identification of CSF-cNs in vitro. We first established a protocol for in vitro culture of CSF-cNs from the cervical spinal cord of mice within 24 h after birth. Polycystic kidney disease 2-like 1 (PKD2L1) cells were isolated by fluorescence-activated cell sorting and expressed the neuron marker β-tubulin III and CSF-cNs marker GABA. Intriguingly, PKD2L1 cells formed neurosphere and expressed neural stem cell markers Nestin, Sox2 and GFAP. Thus, our research provided culture and isolation of CSF-cNs and this facilitate the investigation the CSF-cNs function in vitro.

Citing Articles

Roles of cerebrospinal fluid-contacting neurons as potential neural stem cells in the repair and regeneration of spinal cord injuries.

Xiong Y, Pi W, Zhao W, Shi W, Yan W, Yang H Front Cell Dev Biol. 2024; 12:1426395.

PMID: 38983786 PMC: 11231923. DOI: 10.3389/fcell.2024.1426395.

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