Acceleration of the Deamination of Cytosine Through Photo-Crosslinking
Overview
Overview
Journal
Curr Issues Mol Biol
Publisher
MDPI
Specialty
Molecular Biology
Date
2023 Jun 27
PMID
37367047
Authors
Authors
Affiliations
Affiliations
Soon will be listed here.
Abstract
Herein, we report the major factor for deamination reaction rate acceleration, i.e., hydrophilicity, by using various 5-substituted target cytosines and by carrying out deamination at high temperatures. Through substitution of the groups at the 5'-position of the cytosine, the effect of hydrophilicity was understood. It was then used to compare the various modifications of the photo-cross-linkable moiety as well as the effect of the counter base of the cytosine to edit both DNA and RNA. Furthermore, we were able to achieve cytosine deamination at 37 °C with a half-life in the order of a few hours.
References
1.
Henczi M, Nagy J, Weaver D
. Determination of octanol-water partition coefficients by an HPLC method for anticonvulsant structure-activity studies. J Pharm Pharmacol. 1995; 47(4):345-7.
DOI: 10.1111/j.2042-7158.1995.tb05807.x.
View
2.
Bird A
. CpG-rich islands and the function of DNA methylation. Nature. 1986; 321(6067):209-13.
DOI: 10.1038/321209a0.
View
3.
Frommer M, McDonald L, Millar D, Collis C, Watt F, GRIGG G
. A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proc Natl Acad Sci U S A. 1992; 89(5):1827-31.
PMC: 48546.
DOI: 10.1073/pnas.89.5.1827.
View
4.
Lamont P, Surtees R, Woodward C, Leonard J, Wood N, Harding A
. Clinical and laboratory findings in referrals for mitochondrial DNA analysis. Arch Dis Child. 1998; 79(1):22-7.
PMC: 1717633.
DOI: 10.1136/adc.79.1.22.
View
5.
Iwase R, Namba M, Yamaoka T, Murakami A
. Gene regulation by decoy approach (I): synthesis and properties of photo-crosslinked oligonucleotides. Nucleic Acids Symp Ser. 1997; (37):203-4.
View