DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy

Overview

Journal ACS Nano

Publisher American Chemical Society

Specialty Biotechnology

Date 2023 Jun 21

PMID 37341451

Authors

Xiaojin Wang

Mikkel B Liisberg

Georgia L Nolt

Xu Fu

Cecilia Cerretani

Lan Li

Lance A Johnson

Tom Vosch

Christopher I Richards

Affiliations

Soon will be listed here.

Abstract

Unraveling the transport of drugs and nanocarriers in cerebrovascular networks is important for pharmacokinetic and hemodynamic studies but is challenging due to the complexity of sensing individual particles within the circulatory system of a live animal. Here, we demonstrate that a DNA-stabilized silver nanocluster (DNA-AgNC) that emits in the first near-infrared window upon two-photon excitation in the second NIR window can be used for multiphoton fluorescence correlation spectroscopy for the measurement of cerebral blood flow rates in live mice with high spatial and temporal resolution. To ensure bright and stable emission during experiments, we loaded DNA-AgNCs into liposomes, which served the dual purposes of concentrating the fluorescent label and protecting it from degradation. DNA-AgNC-loaded liposomes enabled the quantification of cerebral blood flow velocities within individual vessels of a living mouse.

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