Effects of Interleukin-23 on the Activation of Mucosal-associated Invariant T Cells from Oral Lichen Planus

Overview

Journal J Int Med Res

Publisher Sage Publications

Specialty General Medicine

Date 2023 Jun 21

PMID 37340721

Authors

Fang Wang

Xiu-Li Zhang

Jing-Ya Yang

Gang Zhou

Affiliations

Soon will be listed here.

Abstract

Objective: Oral lichen planus (OLP) is a T cell-mediated inflammatory condition in the oral cavity. Mucosal-associated invariant T (MAIT) cells are gaining more relevance in immune diseases because they can be activated by cytokines without T cell receptor stimulation. Herein, we tested the effect of interleukin-23 (IL-23) on the activation status of OLP MAIT cells.

Methods: Peripheral blood mononuclear cells (PBMCs) isolated from OLP patients were stimulated by IL-23 in the absence or presence of phorbol myristate acetate (PMA) and ionomycin. The activation status of MAIT cells was analyzed by flow cytometry after staining for CD3, CD4, CD8, CD161, TCR Vα7.2, and CD69.

Results: The fraction of MAIT cells in OLP peripheral blood was approximately 0.38% to 3.97%, and CD8 subpopulations overwhelmed CD4 cells. The mean percentages of OLP MAIT cells in PBMCs and CD8MAIT cells in MAIT cells were approximately 40%. PMA and ionomycin significantly increased CD69 expression on OLP T cells, MAIT cells, and CD8MAIT cells. Cells with enhanced activation had different responsiveness to exogenous IL-23, showing increased CD69 expression on OLP T cells, decreased CD69 on OLP CD8MAIT cells, and no significant change on OLP MAIT cells.

Conclusions: IL-23 showed different effects on the activation status of OLP MAIT cells and CD8MAIT cells.

Citing Articles

MAIT cells modulating the oral lichen planus immune microenvironment: a cellular crosstalk perspective.

Mi Q, Wu X, Chen Y, Meng W Inflamm Res. 2025; 74(1):10.

PMID: 39762617 DOI: 10.1007/s00011-024-01990-6.

References

Yang J, Wang F, Zhou G . Characterization and function of circulating mucosal-associated invariant T cells and γδT cells in oral lichen planus. J Oral Pathol Med. 2021; 51(1):74-85. DOI: 10.1111/jop.13250. View

Tao H, Pan Y, Chu S, Li L, Xie J, Wang P . Differential controls of MAIT cell effector polarization by mTORC1/mTORC2 via integrating cytokine and costimulatory signals. Nat Commun. 2021; 12(1):2029. PMC: 8016978. DOI: 10.1038/s41467-021-22162-8. View

Ke Y, Dang E, Shen S, Zhang T, Qiao H, Chang Y . Semaphorin4D Drives CD8 T-Cell Lesional Trafficking in Oral Lichen Planus via CXCL9/CXCL10 Upregulations in Oral Keratinocytes. J Invest Dermatol. 2017; 137(11):2396-2406. DOI: 10.1016/j.jid.2017.07.818. View

Zhou G, Xia K, Du G, Chen X, Xu X, Lu R . Activation of nuclear factor-kappa B correlates with tumor necrosis factor-alpha in oral lichen planus: a clinicopathologic study in atrophic-erosive and reticular form. J Oral Pathol Med. 2009; 38(7):559-64. DOI: 10.1111/j.1600-0714.2009.00779.x. View

Hinks T, Zhang X . MAIT Cell Activation and Functions. Front Immunol. 2020; 11:1014. PMC: 7267072. DOI: 10.3389/fimmu.2020.01014. View

Catala-Rabasa A, Ndagire D, Sabio J, Fedetz M, Matesanz F, Alcina A . High ACSL5 transcript levels associate with systemic lupus erythematosus and apoptosis in Jurkat T lymphocytes and peripheral blood cells. PLoS One. 2011; 6(12):e28591. PMC: 3232234. DOI: 10.1371/journal.pone.0028591. View

Carrozzo M, Porter S, Mercadante V, Fedele S . Oral lichen planus: A disease or a spectrum of tissue reactions? Types, causes, diagnostic algorhythms, prognosis, management strategies. Periodontol 2000. 2019; 80(1):105-125. DOI: 10.1111/prd.12260. View

Stankov K, Bogdanovic G, Stankov S, Draskovic D, Grubor-Lajsic G, Spasic M . Expression analysis of genes involved in apoptosis, proliferation and endoplasmic reticulum stress in ionomycin/PMA treated Jurkat cells. J BUON. 2012; 17(2):369-76. View

Wang H, Luo Z, Lei L, Sun Z, Zhou M, Dan H . Interaction between oral lichen planus and chronic periodontitis with Th17-associated cytokines in serum. Inflammation. 2013; 36(3):696-704. DOI: 10.1007/s10753-013-9594-2. View

10.

Rodriguez-Cerdeira C, Sanchez-Blanco E, Molares-Vila A . Clinical application of development of nonantibiotic macrolides that correct inflammation-driven immune dysfunction in inflammatory skin diseases. Mediators Inflamm. 2012; 2012:563709. PMC: 3507315. DOI: 10.1155/2012/563709. View

11.

Tsiogkas S, Mavropoulos A, Skyvalidas D, Patrikiou E, Ntavari N, Daponte A . Delphinidin diminishes in vitro interferon-γ and interleukin-17 producing cells in patients with psoriatic disease. Immunol Res. 2021; 70(2):161-173. DOI: 10.1007/s12026-021-09251-y. View

12.

Cheng Y, Gould A, Kurago Z, Fantasia J, Muller S . Diagnosis of oral lichen planus: a position paper of the American Academy of Oral and Maxillofacial Pathology. Oral Surg Oral Med Oral Pathol Oral Radiol. 2016; 122(3):332-54. DOI: 10.1016/j.oooo.2016.05.004. View

13.

Roopashree M, Gondhalekar R, Shashikanth M, George J, Thippeswamy S, Shukla A . Pathogenesis of oral lichen planus--a review. J Oral Pathol Med. 2010; 39(10):729-34. DOI: 10.1111/j.1600-0714.2010.00946.x. View

14.

von Seth E, Zimmer C, Reuterwall-Hansson M, Barakat A, Arnelo U, Bergquist A . Primary sclerosing cholangitis leads to dysfunction and loss of MAIT cells. Eur J Immunol. 2018; 48(12):1997-2004. DOI: 10.1002/eji.201847608. View

15.

Maggi L, Santarlasci V, Capone M, Peired A, Frosali F, Crome S . CD161 is a marker of all human IL-17-producing T-cell subsets and is induced by RORC. Eur J Immunol. 2010; 40(8):2174-81. DOI: 10.1002/eji.200940257. View

16.

Wang F, Zhang J, Zhou G . 2-Deoxy-D-glucose impedes T cell-induced apoptosis of keratinocytes in oral lichen planus. J Cell Mol Med. 2021; 25(21):10257-10267. PMC: 8572795. DOI: 10.1111/jcmm.16964. View

17.

Wang H, Kjer-Nielsen L, Shi M, DSouza C, Pediongco T, Cao H . IL-23 costimulates antigen-specific MAIT cell activation and enables vaccination against bacterial infection. Sci Immunol. 2019; 4(41). DOI: 10.1126/sciimmunol.aaw0402. View

18.

DeAngelis L, Cirillo N, McCullough M . The immunopathogenesis of oral lichen planus-Is there a role for mucosal associated invariant T cells?. J Oral Pathol Med. 2019; 48(7):552-559. DOI: 10.1111/jop.12898. View

19.

Wang H, Zhang D, Han Q, Zhao X, Zeng X, Xu Y . Role of distinct CD4(+) T helper subset in pathogenesis of oral lichen planus. J Oral Pathol Med. 2015; 45(6):385-93. DOI: 10.1111/jop.12405. View

20.

Lu R, Zhang J, Sun W, Du G, Zhou G . Inflammation-related cytokines in oral lichen planus: an overview. J Oral Pathol Med. 2013; 44(1):1-14. DOI: 10.1111/jop.12142. View