Use of the Biotin-avidin System for Detecting a Broad Range of Serologically Related Plant Viruses by ELISA

Overview

Journal J Virol Methods

Specialty Microbiology

Date 1986 May 1

PMID 3734012

Citations 7

Authors

M Zrein

J Burckard

M H Van Regenmortel

Affiliations

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Abstract

The biotin-avidin detection system was used in direct and indirect ELISA for detecting a broad range of serologically related tobamoviruses. When compared to standard ELISA procedures that use antibodies labelled with alkaline phosphatase, the biotin-avidin system increased the assay sensitivity and allowed a wider range of related viral serotypes to be detected.

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