Toxic Effect of Callistemon Viminalis Essential Oil on the Ovary of Engorged Rhipicephalus Microplus Female Ticks (Acari: Ixodidae)

Overview

Journal Exp Appl Acarol

Specialties Biology
Parasitology

Date 2023 Jun 20

PMID 37338639

Authors

Aline Chaves Reis

Isaac Filipe Moreira Konig

Graziela Herminia Andrade Mendonca

Allan da Silva Lunguinho

Maria das Gracas Cardoso

Rafael Neodini Remedio

Affiliations

Soon will be listed here.

Abstract

Rhipicephalus microplus is the main ectoparasite of cattle and its parasitism can reduce weight gain of hosts, in addition to causing anemia, increasing the risk of myiasis, and resulting in the transmission of Babesia bovis and Anaplasma marginale, among other pathogens. The use of synthetic chemicals plays an important role in controlling these ticks. However, its frequent and indiscriminate use has contributed to the selection of resistant strains, resulting in greater interest in the search for natural-origin products. The shrub Callistemon viminalis (Myrtaceae), also known as weeping bottlebrush, is known for its antibacterial, acaricidal, repellent, and antifungal activities, but there are no reports in the literature about its effects on the internal morphology of ticks. This study aimed to extract and characterize the essential oil obtained from the leaves of C. viminalis. Additionally, its effects on the ovary morphology of engorged R. microplus were assessed through histological, histochemical, and morphometric techniques. Exposure to C. viminalis caused dose-dependent morphological changes, such as cellular alterations in the epithelial layer lining the ovary lumen and the pedicel, irregularity of the chorion and oocyte shape, changes in protein and carbohydrate content, decrease in oocyte size, reduction in the size of the nucleus as well as cytoplasmic and nucleolar vacuolation. Thus, C. viminalis essential oil exhibited a toxic effect on the reproductive system of R. microplus, which may result in the reproductive impairment of this tick species.

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