Lactonase-mediated Inhibition of Quorum Sensing Largely Alters Phenotypes, Proteome, and Antimicrobial Activities in E264

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2023 Jun 19

PMID 37333853

Authors

Melanie Gonzales

Laure Plener

Jean Armengaud

Nicholas Armstrong

Eric Chabriere

David Daude

Affiliations

Soon will be listed here.

Abstract

Introduction: is a study model for , a highly virulent pathogen, known to be the causative agent of melioidosis and a potential bioterrorism agent. These two bacteria use an (acyl-homoserine lactone) AHL-mediated quorum sensing (QS) system to regulate different behaviors including biofilm formation, secondary metabolite productions, and motility.

Methods: Using an enzyme-based quorum quenching (QQ) strategy, with the lactonase Pox having the best activity on AHLs, we evaluated the importance of QS in by combining proteomic and phenotypic analyses.

Results: We demonstrated that QS disruption largely affects overall bacterial behavior including motility, proteolytic activity, and antimicrobial molecule production. We further showed that QQ treatment drastically decreases bactericidal activity against two bacteria ( and ), while a spectacular increase in antifungal activity was observed against fungi and yeast (, and ).

Discussion: This study provides evidence that QS is of prime interest when it comes to understanding the virulence of species and developing alternative treatments.

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