Genetics and Marker-assisted Breeding for Sex Expression in Cucumber

Overview

Journal Front Genet

Date 2023 Jun 16

PMID 37323678

Authors

R K Dhall

Harleen Kaur

Pooja Manchanda

Eshanee

Affiliations

Soon will be listed here.

Abstract

Cucumber is an important vegetable crop that provides an accessible draft genome, which has significantly expedited research in various fields of molecular genetics. Cucumber breeders have been employing various methodologies to improve the yield and quality of the crop. These methodologies comprise enhancement of disease resistance, use of gynoecious sex types and their association with parthenocarpy, alterations in plant architecture, and enhancement of genetic variability. The genetics of sex expression are a complex trait in cucumbers but are very significant for the genetic improvement of cucumber crop. This review comprises an explanation of the current status of gene(s) involvement and its expression studies, the inheritance of genes, molecular markers, and genetic engineering associated with sex determination, as well as a discussion of the role of ethylene in sex expression and sex-determining genes of the ACS family. There is no doubt that gynoecy is an important trait among all sex forms of cucumber for heterosis breeding, but if it is associated with parthenocarpy, fruit yield can be enhanced to a greater extent under favorable conditions. However, little information is available with regard to parthenocarpy in gynoecious-type cucumber. This review sheds light on the genetics and molecular mapping of sex expression and could be beneficial especially to cucumber breeders and other scientists working on crop improvement via traditional and molecular assistant approaches.

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References

Gremski K, Ditta G, Yanofsky M . The HECATE genes regulate female reproductive tract development in Arabidopsis thaliana. Development. 2007; 134(20):3593-601. DOI: 10.1242/dev.011510. View

Fazio G, Staub J, Stevens M . Genetic mapping and QTL analysis of horticultural traits in cucumber ( Cucumis sativus L.) using recombinant inbred lines. Theor Appl Genet. 2003; 107(5):864-74. DOI: 10.1007/s00122-003-1277-1. View

Dijkhuizen A, Meglic V, Staub J, Havey M . Linkages among RFLP, RAPD, isozyme, disease-resistance, and morphological markers in narrow and wide crosses of cucumber. Theor Appl Genet. 2013; 89(1):42-8. DOI: 10.1007/BF00226980. View

Boualem A, Troadec C, Kovalski I, Sari M, Perl-Treves R, Bendahmane A . A conserved ethylene biosynthesis enzyme leads to andromonoecy in two cucumis species. PLoS One. 2009; 4(7):e6144. PMC: 2701604. DOI: 10.1371/journal.pone.0006144. View

Zhu W, Huang L, Chen L, Yang J, Wu J, Qu M . A High-Density Genetic Linkage Map for Cucumber (Cucumis sativus L.): Based on Specific Length Amplified Fragment (SLAF) Sequencing and QTL Analysis of Fruit Traits in Cucumber. Front Plant Sci. 2016; 7:437. PMC: 4835494. DOI: 10.3389/fpls.2016.00437. View

Woycicki R, Witkowicz J, Gawronski P, Dabrowska J, Lomsadze A, Pawelkowicz M . The genome sequence of the North-European cucumber (Cucumis sativus L.) unravels evolutionary adaptation mechanisms in plants. PLoS One. 2011; 6(7):e22728. PMC: 3145757. DOI: 10.1371/journal.pone.0022728. View

Yamasaki S, Fujii N, Takahashi H . The ethylene-regulated expression of CS-ETR2 and CS-ERS genes in cucumber plants and their possible involvement with sex expression in flowers. Plant Cell Physiol. 2000; 41(5):608-16. DOI: 10.1093/pcp/41.5.608. View

Zhang Y, Zhao G, Li Y, Mo N, Zhang J, Liang Y . Transcriptomic Analysis Implies That GA Regulates Sex Expression via Ethylene-Dependent and Ethylene-Independent Pathways in Cucumber ( L.). Front Plant Sci. 2017; 8:10. PMC: 5243814. DOI: 10.3389/fpls.2017.00010. View

Shifriss O, George W, Quiones J . Gynodioecism in Cucumbers. Genetics. 1964; 49(2):285-91. PMC: 1210570. DOI: 10.1093/genetics/49.2.285. View

10.

Song K, Shin Y, Jung M, Subramaniyam S, Lee K, Oh E . Chromosome-Scale Genome Assemblies of Two Korean Cucumber Inbred Lines. Front Genet. 2021; 12:733188. PMC: 8640492. DOI: 10.3389/fgene.2021.733188. View

11.

Martin A, Troadec C, Boualem A, Rajab M, Fernandez R, Morin H . A transposon-induced epigenetic change leads to sex determination in melon. Nature. 2009; 461(7267):1135-8. DOI: 10.1038/nature08498. View

12.

Zhou Y, Ahammed G, Wang Q, Wu C, Wan C, Yang Y . Transcriptomic insights into the blue light-induced female floral sex expression in cucumber (Cucumis sativus L.). Sci Rep. 2018; 8(1):14261. PMC: 6155147. DOI: 10.1038/s41598-018-32632-7. View

13.

Trebitsh T, Staub J, Oneill S . Identification of a 1-aminocyclopropane-1-carboxylic acid synthase gene linked to the female (F) locus that enhances female sex expression in cucumber. Plant Physiol. 1997; 113(3):987-95. PMC: 158220. DOI: 10.1104/pp.113.3.987. View

14.

Li Z, Huang S, Liu S, Pan J, Zhang Z, Tao Q . Molecular isolation of the M gene suggests that a conserved-residue conversion induces the formation of bisexual flowers in cucumber plants. Genetics. 2009; 182(4):1381-5. PMC: 2728875. DOI: 10.1534/genetics.109.104737. View

15.

Knopf R, Trebitsh T . The female-specific Cs-ACS1G gene of cucumber. A case of gene duplication and recombination between the non-sex-specific 1-aminocyclopropane-1-carboxylate synthase gene and a branched-chain amino acid transaminase gene. Plant Cell Physiol. 2006; 47(9):1217-28. DOI: 10.1093/pcp/pcj092. View

16.

Pawelkowicz M, Pryszcz L, Skarzynska A, Woycicki R, Posyniak K, Rymuszka J . Comparative transcriptome analysis reveals new molecular pathways for cucumber genes related to sex determination. Plant Reprod. 2019; 32(2):193-216. PMC: 6500512. DOI: 10.1007/s00497-019-00362-z. View

17.

Bu F, Chen H, Shi Q, Zhou Q, Gao D, Zhang Z . A major quantitative trait locus conferring subgynoecy in cucumber. Theor Appl Genet. 2015; 129(1):97-104. DOI: 10.1007/s00122-015-2612-z. View

18.

Sebastian P, Schaefer H, Telford I, Renner S . Cucumber (Cucumis sativus) and melon (C. melo) have numerous wild relatives in Asia and Australia, and the sister species of melon is from Australia. Proc Natl Acad Sci U S A. 2010; 107(32):14269-73. PMC: 2922565. DOI: 10.1073/pnas.1005338107. View

19.

Mibus H, Tatlioglu T . Molecular characterization and isolation of the F/f gene for femaleness in cucumber ( Cucumis sativus L.). Theor Appl Genet. 2004; 109(8):1669-76. DOI: 10.1007/s00122-004-1793-7. View

20.

Qi J, Liu X, Shen D, Miao H, Xie B, Li X . A genomic variation map provides insights into the genetic basis of cucumber domestication and diversity. Nat Genet. 2013; 45(12):1510-5. DOI: 10.1038/ng.2801. View